Mouse anti-Human Na+/K+ ATPase beta3 Monoclonal Antibody | anti-ATP1B3 antibody

Na+/K+ ATPase beta3 (Extracellular) M025 Antibody

Cat. Num. AAA474468

• Gene Names: ATP1B3; CD298; ATPB-3
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunoprecipitation, Immunocytochemistry
• Purity: Protein G chromatography
• Synonyms: Na+/K+ ATPase beta3; Monoclonal Antibody; Na+/K+ ATPase beta3 (Extracellular) M025 Antibody; Na+/K+ ATPase beta3 (Extracellular); Na+/K+ ATPase beta3 M025; anti-ATP1B3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: M025
• Specificity: Clone M025 mouse monoclonal antibody detects a 40kDa* protein on SDS-PAGE "Native" immunoblots of human A431, LNCaP, MeWo, MDA-MB-231, and MCF7 cells. This antibody does not detect denatured Na+/K+ ATPase beta3 subunit. The antibody works for western blot, immunoprecipitation, ELISA, and immunocytochemistry, as well as detects the beta3 subunit on live cells.
• Purity/Purification: Protein G chromatography
• Form/Format: Mouse monoclonal antibody purified with protein G chromatography is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-ATP1B3 antibody: Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), Immunocytochemistry (ICC)
• Application Notes: ELISA: 1:2000; ICC: 1:100; IP: 1:100; WB: 1:1000
• Immunogen: Clone (M025) was generated from a proprietary antigen related to the native human Na+/K+ ATPase beta3 subunit expressed in MeWo melanoma cell line.
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

Western Blot (WB)

(Western blot of native human MDA-MB-231 (lane 1), and MeWo (lane 2) cell lysates, as well as native human breast (lane 3), lung (lane 4), skin (lane 5), and brain (lane 6) tissues. The blot was probed with anti-Na+/K+ ATPase beta3 (NM0251) at 1:1000.)

Immunocytochemistry (ICC)

(Immunocytochemical labeling of Na+/K+ ATPase beta3 in paraformaldehyde fixed human MeWo melanoma cells. The cells were labeled with mouse monoclonal anti-Na+/K+ ATPase beta3 (clone M025). The antibody was detected using goat anti-mouse Ig DyLight 594.)

Related Product Information for anti-ATP1B3 antibody

The Na+/K+ ATPase is an integral membrane heterodimer belonging to the P-type ATPase family. This ion channel uses the energy derived from ATP hydrolysis to maintain membrane potential by driving Na+ export and K+ import across the plasma membrane. It is composed of a large catalytic alpha subunit and a membrane-spanning auxiliary beta subunit. In humans, the Na+/K+ ATPase is a binary complex of an alpha subunit that has four isoforms (alpha1-alpha4) and a beta-subunit that has three isoforms (beta1, beta2, beta3). Na+/K+ ATPase subunit expression has been shown to be upregulated in cancers, and inhibition of Na+/K+ ATPase activity has anti-cancer effects. The beta3 subunit of Na+/K+ ATPase has increased expression in human gastric cancer tissues and cell lines, and its increased expression level predicts poor patient outcome. beta3 subunit knockdown significantly inhibited cell proliferation, colony-formation ability, migration, and invasion in human gastric carcinoma cell lines.

References

Clausen, MV et al. (2017) Front Physiol. 8:371.
Li, L et al. (2017) Oncotarget. 8(48):84285.
Yoshimura, SH et al. (2008) J Cell Sci. 121:2159.

NCBI and Uniprot Product Information

• NCBI GI #: 4502281
• NCBI GeneID: 483
• NCBI Accession #: NP_001670.1
• NCBI GenBank Nucleotide #: NM_001679.2
• UniProt Accession #: P54709
• Molecular Weight: 31,513 Da
• NCBI Official Full Name: sodium/potassium-transporting ATPase subunit beta-3
• NCBI Official Synonym Full Names: ATPase, Na+/K+ transporting, beta 3 polypeptide
• NCBI Official Symbol: ATP1B3
• NCBI Official Synonym Symbols: CD298; ATPB-3
• NCBI Protein Information: sodium/potassium-transporting ATPase subunit beta-3; sodium pump subunit beta-3; Na, K-ATPase beta-3 polypeptide; sodium/potassium-dependent ATPase beta-3 subunit; sodium/potassium-dependent ATPase subunit beta-3; sodium/potassium-transporting ATPase beta
• UniProt Protein Name: Sodium/potassium-transporting ATPase subunit beta-3
• UniProt Gene Name: ATP1B3
• UniProt Synonym Gene Names: ATPB-3
• UniProt Entry Name: AT1B3_HUMAN

NCBI Description

The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 3 subunit. This gene encodes a beta 3 subunit. A pseudogene exists for this gene, and it is located on chromosome 2. [provided by RefSeq, Jul 2008]

Uniprot Description

ATP1B3: This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The exact function of the beta-3 subunit is not known. Belongs to the X(+)/potassium ATPases subunit beta family.

Protein type: Membrane protein, integral; Transporter

Chromosomal Location of Human Ortholog: 3q23

Cellular Component: melanosome; plasma membrane; caveola; sodium:potassium-exchanging ATPase complex

Molecular Function: ATPase activator activity; sodium:potassium-exchanging ATPase activity; ATPase binding

Biological Process: cellular sodium ion homeostasis; potassium ion import; protein stabilization; transport; positive regulation of ATPase activity; blood coagulation; cellular potassium ion homeostasis; leukocyte migration; transmembrane transport