Mouse HISTONE H1 Monoclonal Antibody | anti-H1 antibody
MOUSE ANTI HUMAN HISTONE H1
Gene Names
H1; leH1
Applications
Immunohistochemistry, ELISA, Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Western Blot
Synonyms
HISTONE H1; Monoclonal Antibody; MOUSE ANTI HUMAN HISTONE H1; anti-H1 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
AE-4
Form/Format
Purified
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Sequence Length
271
Applicable Applications for anti-H1 antibody
Immunohistology Frozen*, ELISA (EIA), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistology Paraffin, Western Blot (WB)
Application Notes
Immunohistology - Frozen: Application Note: Acetone or PFA fixation is recommended
Western Blotting: Maximum Dilution: 1/100
Western Blotting: Maximum Dilution: 1/100
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
Preparation
Preparation
Immunogen
Nuclei from myeloid leukamia biopsy cells.
Histology Positive Control Tissue
Histology Positive Control Tissue
Target Species
Human
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Related Product Information for anti-H1 antibody
Mouse anti Human Histone H1 antibody, clone AE-4 recognizes histone H1.The histone proteins serve as a structural scaffold for the organization of nuclear DNA into chromatin. In eukaryotic cells there are 4 core histone proteins, H2A, H2B, H3 and H4 that assemble to form an octamer round which the DNA is wrapped in a sequence dependant manner. The resulting complex is known as a nucleosome and is the basic subunit of chromatin. Histone H1 acts as a linker and binds to each nucleosome, and by self affinity, links these nucleosomes together resulting in the 30 nm chromatin fiber.In blots of rat brain homogenate a duplet is observed at 27-29 kDa consistent with Histone H1. Speckled staining is observed in the nucleus of normal and malignant cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
histone H1
NCBI Official Symbol
H1
NCBI Official Synonym Symbols
leH1
NCBI Protein Information
histone H1
Protein Family
Research Articles on H1
Similar Products
Product Notes
The H1 (Catalog #AAA211699) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's HISTONE H1 can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen*, ELISA (EIA), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistology Paraffin, Western Blot (WB). Immunohistology - Frozen: Application Note: Acetone or PFA fixation is recommended Western Blotting: Maximum Dilution: 1/100. Researchers should empirically determine the suitability of the H1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HISTONE H1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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