Mouse anti-Human, Mouse hHR23b Monoclonal Antibody | anti-RAD23B antibody

hHR23b Antibody

Cat. Num. AAA9215439

• Gene Names: RAD23B; P58; HR23B; HHR23B
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry
• Synonyms: hHR23b; Monoclonal Antibody; hHR23b Antibody; Purified Mouse Monoclonal Antibody (Mab); hHR 23b; hHR23B; HR 23B; HR23B; mHR 23B; mHR23B; P58; RAD 23B; RAD23 (S. cerevisiae) homolog B; RAD23 homolog B (S. cerevisiae); RAD23 homolog B; RAD23 yeast homolog of B; RAD23B; RD23B_HUMAN; UV excision repair protein RAD23 homolog B; XP C repair complementing complex 58 kDa; XP C repair complementing complex 58 kDa protein; XP C repair complementing protein; XP-C repair-complementing complex 58 kDa protein; XPC repair complementing complex 58 kDa; XPC repair complementing complex 58 kDa protein; XPC repair complementing protein.; anti-RAD23B antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Form/Format: Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
• Sequence Length: 337

• Applicable Applications for anti-RAD23B antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
• Application Notes: WB ~~ 1:1000; ICC ~~ 1:100; IHC ~~ 1:100
• Antigen Source: Human
• Antigen Type: Recombinant Protein
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot detection of hHR23b in A431,K562,Jurkat,C6,3T3 and Hela cell lysates using hHR23b mouse mAb (1:1000 diluted).Predicted band size:58KDa.Observed band size:58KDa.Exposure time:5min.)

Immunocytochemistry (ICC)

(Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-hHR23b antibody (dilution 1:100).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Prostate Cancer using hHR23b mouse mAb (1/100 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).)

Related Product Information for anti-RAD23B antibody

Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome. May play a role in endoplasmic reticulum- associated degradation (ERAD) of misfolded glycoproteins by association with PNGase and delivering deglycosylated proteins to the proteasome. The XPC complex is proposed to represent the first factor bound at the sites of DNA damage and together with other core recognition factors, XPA, RPA and the TFIIH complex, is part of the pre-incision (or initial recognition) complex. The XPC complex recognizes a wide spectrum of damaged DNA characterized by distortions of the DNA helix such as single-stranded loops, mismatched bubbles or single-stranded overhangs. The orientation of XPC complex binding appears to be crucial for inducing a productive NER. XPC complex is proposed to recognize and to interact with unpaired bases on the undamaged DNA strand which is followed by recruitment of the TFIIH complex and subsequent scanning for lesions in the opposite strand in a 5'-to-3' direction by the NER machinery. Cyclobutane pyrimidine dimers (CPDs) which are formed upon UV-induced DNA damage esacpe detection by the XPC complex due to a low degree of structural perurbation. Instead they are detected by the UV-DDB complex which in turn recruits and cooperates with the XPC complex in the respective DNA repair. In vitro, the XPC:RAD23B dimer is sufficient to initiate NER; it preferentially binds to cisplatin and UV-damaged double-stranded DNA and also binds to a variety of chemically and structurally diverse DNA adducts. XPC:RAD23B contacts DNA both 5' and 3' of a cisplatin lesion with a preference for the 5' side. XPC:RAD23B induces a bend in DNA upon binding. XPC:RAD23B stimulates the activity of DNA glycosylases TDG and SMUG1.

References

Masutani C.,et al.EMBO J. 13:1831-1843(1994). Huang X.,et al.J. Androl. 25:363-368(2004). Ota T.,et al.Nat. Genet. 36:40-45(2004). Humphray S.J.,et al.Nature 429:369-374(2004). Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

• NCBI GI #: 347800665
• NCBI GeneID: 5887
• NCBI Accession #: NP_001231653.1
• NCBI GenBank Nucleotide #: NM_001244724.1
• UniProt Accession #: P54727; Q7Z5K8; Q8WUB0; B3KWK8; G5E9P0
• Molecular Weight: 43171
• NCBI Official Full Name: UV excision repair protein RAD23 homolog B isoform 3
• NCBI Official Synonym Full Names: RAD23 homolog B, nucleotide excision repair protein
• NCBI Official Symbol: RAD23B
• NCBI Official Synonym Symbols: P58; HR23B; HHR23B
• NCBI Protein Information: UV excision repair protein RAD23 homolog B
• UniProt Protein Name: UV excision repair protein RAD23 homolog B
• Protein Family: Ubiquitin receptor
• UniProt Gene Name: RAD23B
• UniProt Synonym Gene Names: HR23B; hHR23B; p58
• UniProt Entry Name: RD23B_HUMAN

NCBI Description

The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. This protein was also shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus this protein may be involved in the ubiquitin mediated proteolytic pathway in cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2011]

Uniprot Description

RAD23B: Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome. May play a role in endoplasmic reticulum- associated degradation (ERAD) of misfolded glycoproteins by association with PNGase and delivering deglycosylated proteins to the proteasome. Belongs to the RAD23 family.

Protein type: Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 9q31.2

Cellular Component: nucleoplasm; proteasome complex; cytoplasm; nucleus

Molecular Function: protein binding; damaged DNA binding; polyubiquitin binding; single-stranded DNA binding

Biological Process: nucleotide-excision repair, DNA damage recognition; proteasomal ubiquitin-dependent protein catabolic process; nucleotide-excision repair; regulation of proteasomal ubiquitin-dependent protein catabolic process; nucleotide-excision repair, DNA damage removal; spermatogenesis; DNA repair