Western Blot (WB)
(Western Blot: Sample: Recombinant HGF, Human.)
Mouse anti-Human Hepatocyte Growth Factor (HGF) Monoclonal Antibody | anti-HGF antibody
Monoclonal Antibody to Hepatocyte Growth Factor (HGF)
Immunohistochemistry: 5-20 ug/mL;1:40-160
Immunocytochemistry: 5-20 ug/mL;1:40-160
Optimal working dilutions must be determined by end user
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot: Sample: Recombinant HGF, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant HGF, Human.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein that binds to the hepatocyte growth factor receptor to regulate cell growth, cell motility and morphogenesis in numerous cell and tissue types. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate alpha and beta chains, which form the mature heterodimer. This protein is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. This protein also plays a role in angiogenesis, tumorogenesis, and tissue regeneration. Although the encoded protein is a member of the peptidase S1 family of serine proteases, it lacks peptidase activity. Mutations in this gene are associated with nonsyndromic hearing loss. [provided by RefSeq, Nov 2015]
Uniprot Description
HGF: HGF is a potent mitogen for mature parenchymal hepatocyte cells, seems to be an hepatotrophic factor, and acts as growth factor for a broad spectrum of tissues and cell types. It has no detectable protease activity. Activating ligand for the receptor tyrosine kinase MET by binding and promoting its dimerization. Defects in HGF are the cause of deafness autosomal recessive type 39 (DFNB39). A form of profound prelingual sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Belongs to the peptidase S1 family. Plasminogen subfamily. 6 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell development/differentiation; Hormone; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 7q21.11
Cellular Component: extracellular region; extracellular space; membrane
Molecular Function: chemoattractant activity; growth factor activity; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protein binding; protein heterodimerization activity; protein tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity; serine-type endopeptidase activity
Biological Process: activation of MAPK activity; cell morphogenesis; epithelial to mesenchymal transition; hepatocyte growth factor receptor signaling pathway; hyaluronan metabolic process; liver development; MAPK cascade; mitotic cell cycle; myoblast proliferation; negative regulation of autophagy; negative regulation of cysteine-type endopeptidase activity involved in apoptotic process; negative regulation of inflammatory response; negative regulation of interleukin-6 production; negative regulation of peptidyl-serine phosphorylation; organ regeneration; peptidyl-tyrosine phosphorylation; phosphatidylinositol phosphorylation; phosphoinositide-mediated signaling; platelet degranulation; positive chemotaxis; positive regulation of angiogenesis; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of GTPase activity; positive regulation of interleukin-10 production; positive regulation of myelination; positive regulation of osteoblast differentiation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of transcription from RNA polymerase II promoter; proteolysis; regulation of phosphatidylinositol 3-kinase signaling
Disease: Deafness, Autosomal Recessive 39
Research Articles on HGF
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Product Notes
The HGF hgf (Catalog #AAA2012807) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to Hepatocyte Growth Factor (HGF) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Hepatocyte Growth Factor (HGF) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). Western blotting: 0.5-2 ug/mL;1:400-1600 Immunohistochemistry: 5-20 ug/mL;1:40-160 Immunocytochemistry: 5-20 ug/mL;1:40-160 Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the HGF hgf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hepatocyte Growth Factor (HGF), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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