Hepatitis B Virus X antigen Monoclonal Antibody | anti-X antibody

Hepatitis B Virus X antigen

Cat. Num. AAA8535039

• Reactivity: Human, Mouse, Rat
• Applications: Immunoprecipitation, Western Blot
• Purity: Protein A affinity purified.
• Synonyms: Hepatitis B Virus X antigen; Monoclonal Antibody; anti-X antibody

• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Clone Number: 4G6
• Purity/Purification: Protein A affinity purified.
• Form/Format: 1*TBS (pH7.4), 1%BSA, 40% Glycerol.

• Applicable Applications for anti-X antibody: Western Blot (WB); Immunoprecipitation (IP).
• Application Notes: WB: 1:100-1:1,000; IP: 1–2 ug per 100–500 ug of total protein (1 ml of cell lysate).
• Product Type: Mouse monoclonal IgG1, primary antibodies.
• Immunogen: peptide
• Preparation and Storage: Store at +4 degree C

Related Product Information for anti-X antibody

Hep B (hepatitus B) virus is a member of the Hepadnavirus family that causes an inflammation of the liver, vomiting, jaundice and, sometimes, death. Three major antigens make up different parts of the Hep B virus (HBV): surface antigen (Hep B sAg), an envelope glycoprotein found as membranous aggregates in the sera of individuals infected with HBV; e-antigen (Hep B eAg), which is typically associated with much higher rates of viral replication; and core antigen (Hep B cAg), which encloses the viral genome and makes up the assembled and unassembled variants of the capsid protein. Hep B cAg and Hep B eAg are used primarily in HBV identification, whereas Hep B sAg is used for HBV prevention in vaccines. Hep B viral antigens are primarily expressed in liver. Hep B xAg represents the Hep B virus Xprotein which contributes to human hepatocellular carcinoma metastasis by the upregulation of matrix metalloproteinases.

NCBI and Uniprot Product Information

• NCBI GI #: 82066779
• UniProt Accession #: Q4R1S1
• Molecular Weight: 17kDa
• NCBI Official Full Name: Protein X
• UniProt Protein Name: Protein X
• UniProt Gene Name: X

Uniprot Description

Multifunctional protein that plays a role in silencing host antiviral defenses and promoting viral transcription. Does not seem to be essential for HBV infection. May be directly involved in development of cirrhosis and liver cancer (hepatocellular carcinoma). Most of cytosolic activities involve modulation of cytosolic calcium. The effect on apoptosis is controversial depending on the cell types in which the studies have been conducted. May induce apoptosis by localizing in mitochondria and causing loss of mitochondrial membrane potential. May also modulate apoptosis by binding host CFLAR, a key regulator of the death-inducing signaling complex (DISC). Promotes viral transcription by using the host E3 ubiquitin ligase DDB1 to target the SMC5-SMC6 complex to proteasomal degradation. This host complex would otherwise bind to viral episomal DNA, and prevents its transcription. Moderately stimulates transcription of many different viral and cellular transcription elements. Promoters and enhancers stimulated by HBx contain DNA binding sites for NF-kappa-B, AP-1, AP-2, c-EBP, ATF/CREB, or the calcium-activated factor NF-AT.