Mouse Heat Shock Transcription Factor-1 Monoclonal Antibody | anti-HSF1 antibody

Mouse Anti Human Heat Shock Transcription Factor-1

Cat. Num. AAA140262

• Gene Names: HSF1; HSTF1
• Applications: ELISA, Western Blot
• Purity: HSF1 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Synonyms: Heat Shock Transcription Factor-1; Monoclonal Antibody; Mouse Anti Human Heat Shock Transcription Factor-1; HSF1 Antibody; Mouse Anti Human; HSF-1; HSF1; HSTF-1; HSTF1; Heat shock factor protein 1; Heat shock transcription factor 1; anti-HSF1 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1 heavy chain and kappa light chain
• Clone Number: PJ7F9AT
• Purity/Purification: HSF1 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Form/Format: 1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.
• Sequence Length: 529

• Applicable Applications for anti-HSF1 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: HSF1 antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000~ 2,000. Recommended starting dilution is 1:1,000.
• Immunogen: Anti-human HSF1 mAb, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with recombinant human HSF1 amino acids 1-529 purified from E Coli.
• Preparation and Storage: For periods up to 1 month store at 4 degree C, for longer periods of time, store at -20 degree C. Prevent freeze thaw cycles.; 12 months at -20 degree C. 1 month at 4 degree C.

Related Product Information for anti-HSF1 antibody

Introduction: The transcription of heat-shock genes is rapidly induced after temperature stress in response to environmental changes, and it is also involved in oogenesis, spermatogenesis, and placental development. Hsp90, is a major repressor of HSF1 gene. HSF1 is known for its participation in heat shock response. HSF1 protein regulates the transcription of hundreds of targets, such as genes involved in protein folding, detoxification, energy generation, carbohydrate metabolism, and cell wall organization. HSF1 binds to MTA1 in vitro & in breast carcinoma. Suppression of estrogen-dependent transcription may contribute to role of HSF1 in cancer. Human cancer lines of diverse origins express higher dependence on HSF1 function to maintain proliferation and survival than their non-transformed counterparts. HSF1 induces anticoagulation and relaxation factors in vascular endothelial cells and could therefore be used to treat cardiovascular disease. HSF1 is directly involved in the regulation of HO1 havi

Product Categories/Family for anti-HSF1 antibody

Monoclonal Antibodies; Anti-Human Heat Shock Proteins; Monoclonal Antibodies; Anti Human Heat Shock Protein

NCBI and Uniprot Product Information

• NCBI GI #: 5031767
• NCBI GeneID: 3297
• NCBI Accession #: NP_005517.1
• NCBI GenBank Nucleotide #: NM_005526.2
• Molecular Weight: 52,881 Da
• NCBI Official Full Name: heat shock factor protein 1
• NCBI Official Synonym Full Names: heat shock transcription factor 1
• NCBI Official Symbol: HSF1
• NCBI Official Synonym Symbols: HSTF1
• NCBI Protein Information: heat shock factor protein 1; HSF 1; HSTF 1
• UniProt Protein Name: Heat shock factor protein 1
• Protein Family: Heat shock factor protein
• UniProt Gene Name: HSF1
• UniProt Synonym Gene Names: HSTF1; HSF 1; HSTF 1
• UniProt Entry Name: HSF1_HUMAN

NCBI Description

The product of this gene is a heat-shock transcription factor. Transcription of heat-shock genes is rapidly induced after temperature stress. Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

HSF1: a transcription factor that specifically binds heat shock promoter elements (HSE) and activates transcription. Induced in response to heat, heavy metals, and oxidative stress. In higher eukaryotes, HSF is unable to bind to HSEs unless the cells are stressed. Becomes phosphorylated in response to stress, forming homotrimers that bind DNA and activate transcription. Phosphorylation by PLK1 enhances nuclear translocation, and phosphorylation by CaMKII enhances transactivation. Phosphorylation by GSK3 and ERK1 induces binding by 14-3-3 and sequestration in the cytoplasm. In addition, during attenuation from the heat shock response, HSF1 is repressed by direct binding of Hsp70, HSP40, and HSF binding protein 1 (HSBP1). Four alternatively spliced isoforms have been described.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: nucleoplasm; protein complex; pronucleus; cytoplasm; cytosol

Molecular Function: protein binding; chromatin binding; transcription factor activity

Biological Process: negative regulation of cell proliferation; embryonic placenta development; embryonic process involved in female pregnancy; mRNA transcription; female meiosis; positive regulation of transcription from RNA polymerase II promoter; response to lipopolysaccharide; defense response; spermatogenesis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of tumor necrosis factor production; positive regulation of multicellular organism growth; protein amino acid phosphorylation