Western Blot (WB)
(Anti-PNMT rabbit polyclonal antibody at 1:500 dilution Lane A: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:31 kDa)
Rabbit anti-Human PNMT Polyclonal Antibody | anti-PNMT antibody
Anti-PNMT Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
PNMT; Polyclonal Antibody; Anti-PNMT Antibody; Rabbit Polyclonal; PNMT Antibody; Rabbit PAb; Antigen Affinity Purified; phenylethanolamine N-methyltransferase; Anti-PENT Antibody; Anti-PNMTase Antibody; anti-PNMT antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human PNMT
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-PNMT antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IP: 1-5uL/mg of lysate
IP: 1-5uL/mg of lysate
Immunogen
E Coli-derived Human PNMT fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human PNMT fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-PNMT rabbit polyclonal antibody at 1:500 dilution Lane A: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:31 kDa)
Western Blot (WB)
(Anti-PNMT rabbit polyclonal antibody at 1:500 dilution Lane A: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:31 kDa)
Immunoprecipitation (IP)
(PNMT was immunoprecipitated using: Lane A:0.5 mg K562 Whole Cell Lysate 4 uL anti-PNMT rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PNMT rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 30 kDa Observed band size :30 kDa)
Immunoprecipitation (IP)
(PNMT was immunoprecipitated using: Lane A:0.5 mg K562 Whole Cell Lysate 4 uL anti-PNMT rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PNMT rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 30 kDa Observed band size :30 kDa)
Related Product Information for anti-PNMT antibody
PNMT (phenylethanolamine-N-methyl-transferase) is the enzyme that catalyzes the formation of epinephrine from norepinephrine and thus serves as a marker of adrenergic cells. PNMT is the final enzyme in the catecholamine synthesizing cascade that converts noradrenaline (NA) to adrenaline (ADR). Both of these catecholamines are physiologically important hormones and neurotransmitters in mammals with profound influence on the activity of the cardiovascular system. PNMT is the rate-limiting enzyme in the synthesis of epinephrine and a specific marker for adrenergic neurons. The activity of human PNMT is reduced in the neurons of those cells in many subcortical areas of the brain that are known to undergo neurodegeneration in Alzheimer's disease (AD).
References
Tillinger A, et al. (2006) Modulation by 6-hydroxydopamine of expression of the phenylethanolamine n-methyltransferase (pnmt) gene in the rat heart during immobilization stress. Stress 9 (4): 207-213.Owji A, et al. (2013) Therapeutic potential of pnmt+ primer cells for neuro/myocardial regeneration. Am J Stem Cells 2 (3): 137-154.Mann MB, et al. (2001) Phenylethanolamine n-methyltransferase (pnmt) gene and early-onset alzheimer disease. Am J Med Genet 105 (4): 312-316.Burke WJ, et al. (1990) Evidence for decreased transport of pnmt protein in advanced alzheimer's disease. J Am Geriatr Soc 38 (12): 1275-1282.
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Product Notes
The PNMT (Catalog #AAA8109552) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PNMT Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PNMT can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:500-1:2000 IP: 1-5uL/mg of lysate. Researchers should empirically determine the suitability of the PNMT for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PNMT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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