Mouse Heat Shock Protein 70 / HSPA1A Monoclonal Antibody | anti-HSPA1A antibody

Anti-Heat Shock Protein 70 / HSPA1A Antibody (clone 5F3) IHC-plus

Cat. Num. AAA249368

• Gene Names: HSPA1A; HSP72; HSPA1; HSP70I; HSP70-1; HSP70.1; HSP70-1A; HEL-S-103
• Reactivity: Dog, Human, Monkey
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, Flow Cytometry, Functional Assay
• Purity: Protein A/G purified
• Synonyms: Heat Shock Protein 70 / HSPA1A; Monoclonal Antibody; Anti-Heat Shock Protein 70 / HSPA1A Antibody (clone 5F3) IHC-plus; Mouse Monoclonal [clone 5F3] (IgG1) to Human Heat Shock Protein 70 / HSPA1A; Human Heat Shock Protein 70 / HSPA1A; anti-HSPA1A antibody

• Host: Mouse
• Reactivity: Dog, Human, Monkey
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 5F3
• Specificity: Human HSPA1A / HSP72
• Purity/Purification: Protein A/G purified
• Form/Format: PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
• Concentration: 0.69 mg/ml (varies by lot)
• Sequence Length: 641

• Applicable Applications for anti-HSPA1A antibody: Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Flow Cytometry (1:100); IF (1:100); IHC-P (10 ug/ml); WB (1:4000)
• Immunogen: Full length human recombinant protein of human HSPA1A (NP_005336) produced in HEK293T cell.
• Immunogen Description: Full length human recombinant protein of human HSPA1A (NP_005336) produced in HEK293T cell.
• Immunogen Type: Recombinant protein
• Target Species: Human
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry - Paraffin (IHC)

(Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunofluorescence (IF)

(Immunofluorescent staining of HepG2 cells using anti-HSPA1A mouse monoclonal antibody.)

Immunofluorescence (IF)

(Anti-HSPA1A mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HSPA1A.)

Western Blot (WB)

(HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPA1A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPA1A.)

Western Blot (WB)

(Western blot of extracts (35 ug) from 9 different cell lines by using anti-HSPA1A monoclonal antibody.)

Flow Cytometry (FC/FACS)

(Flow cytometry of HeLa cells, using anti-HSPA1A antibody, (Red) compared to a nonspecific negative control antibody (Blue).)

Flow Cytometry (FC/FACS)

(Flow cytometry of Jurkat cells, using anti-HSPA1A antibody, (Red) compared to a nonspecific negative control antibody (Blue).)

Related Product Information for anti-HSPA1A antibody

In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins.

NCBI and Uniprot Product Information

• NCBI GI #: 194248072
• NCBI GeneID: 3303
• NCBI Accession #: NP_005336.3
• NCBI GenBank Nucleotide #: NM_005345.5
• Molecular Weight: 63,937 Da
• NCBI Official Full Name: heat shock 70 kDa protein 1A
• NCBI Official Synonym Full Names: heat shock protein family A (Hsp70) member 1A
• NCBI Official Symbol: HSPA1A
• NCBI Official Synonym Symbols: HSP72; HSPA1; HSP70I; HSP70-1; HSP70.1; HSP70-1A; HEL-S-103
• NCBI Protein Information: heat shock 70 kDa protein 1A
• UniProt Protein Name: Heat shock 70 kDa protein 1A
• Protein Family: Heat shock 70 kDa protein
• UniProt Gene Name: HSPA1A
• UniProt Synonym Gene Names: HSPA1; HSX70; HSP70.1
• UniProt Entry Name: HS71A_HUMAN

NCBI Description

This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq, Jul 2008]

Uniprot Description

HSP70: a critical chaperone protein that has a high affinity for unfolded polypeptide chains. It binds extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In cooperation with other chaperones, hsp70 stabilizes preexistent proteins against aggregation and mediates the folding of newly translated polypeptides in the cytosol as well as within organelles. Mitochondrial HSP70 is crucial to the import process: mutant forms of HSP70 fail to import precursor proteins. Is anti-apoptotic in sympathetic neurones and mediates this effect primarily by suppressing c-Jun transcriptional signalling. Interacts with tau protein and mediates proper folding of tau. Can promote the degradation of tau protein. Triptolide, a potential therapeutic agent for progression/metastasis of pancreatic cancer, causes pancreatic cancer cell death by induction of apoptosis, an effect mediated by the inhibition of HSP70.

Protein type: Heat shock protein; Motility/polarity/chemotaxis; Chaperone

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: centriole; cytoplasm; cytosol; focal adhesion; inclusion body; mitochondrion; nucleoplasm; perinuclear region of cytoplasm; ubiquitin ligase complex

Molecular Function: ATP binding; ATPase activity; ATPase activity, coupled; enzyme binding; G-protein-coupled receptor binding; heat shock protein binding; histone deacetylase binding; protein binding; receptor binding; ubiquitin protein ligase binding; unfolded protein binding; viral receptor activity

Biological Process: activation of NF-kappaB transcription factor; ATP metabolic process; entry of virus into host cell; gene expression; negative regulation of protein ubiquitination; positive regulation of interleukin-8 production; protein refolding; protein stabilization; regulation of mRNA stability; regulation of protein ubiquitination