Mouse Heat Shock Protein 60 Monoclonal Antibody | anti-HSP60 antibody

Heat Shock Protein 60 (HSP60, GroEL, Chaperonin 60, cpn60) (BSA & Azide Free) (AP)

Cat. Num. AAA6265777

• Reactivity: Chicken, Hamster, Human, Mammal, Mouse, Rat
• Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by Protein G Affinity Chromatography
• Synonyms: Heat Shock Protein 60; Monoclonal Antibody; Heat Shock Protein 60 (HSP60; GroEL; Chaperonin 60; cpn60) (BSA & Azide Free) (AP); anti-HSP60 antibody

• Host: Mouse
• Reactivity: Chicken, Hamster, Human, Mammal, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 0.N.322
• Specificity: Recognizes human Heat Shock Protein 60 at ~60kD. Species Crossreactivity: mouse, rat, hamster, mammal and chicken. Does not react with bacteria, helminths or spinach.
• Purity/Purification: Purified by Protein G Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-HSP60 antibody: ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Immunogen: Recombinant HSP60 protein. Cellular Localization: Cytoplasmic. Epitope: aa383-447
• Conjugate: AP
• Preparation and Storage: Store at 4 degree C. Do Not Freeze.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human breast carcinoma stained H1830-75X using peroxidase-conjugate and AEC chromogen. Note: intense cytoplasmic staining of tumor cells.)

Product Categories/Family for anti-HSP60 antibody

Antibodies; Heat Shock Proteins

References

1. Hirata, D., Hirai, I., Iwamoto, M., Yoshio, T., Takeda, A., Masuyama, J.I., Mimori, A., Kano, S., Minota, S., Preferential binding with Escherichia coli hsp60 of antibodies prevalent in sera from patients with rheumatoid arthritis. Clinical Immunology and Immunopathology 82(2): 141-8 (1997, Feb). 2. Mobius, J., Groos, S., Meinhardt, A., Seitz, J., Differential distribution of the mitochondrial heat-shock protein 60 in rat gastrointestinal tract. Cell and Tissue Research 287(2): 343-50 (1997, Feb). 3. Soltys, B.J., Gupta, R.S., Cell surface localization of the 60kD heat shock chaperonin protein (hsp60) in mammalian cells. Cell Biology International 21(5): 315-20 (1997, May). 4. Werner, A., Meinhardt, A., Seitz, J., Bergmann, M., Distribution of heat-shock protein 60 immunoreactivity in testes of infertile men. Cell and Tissue Research 288(3) :539-44 (1997, Jun). 5. Alevy, Y.G., Brennan, D., Durriya, S., Howard, T., Mohanakumar, T., Increased expression of the HDJ-2 heat shock protein in biopsies of human rejected kidney. Transplantation 61(6): 963-7 (1996). 6. Barker, R.N., Wells, A.D., Ghoraishian, M., Easterfield, A.J., Hitsumoto, Y., Elson, C.J., Thompson, S.J., Expression of mammalian 60kD heat shock protein in the joints of mice with pristane-induced arthritis. Clinical and Experimental Immunology 103(1): 83-8 (1996, Jan). 7. Beimnet, K., Soderstrom, K., Jindal, S., Gronberg, A., Frommel, D., Kiessling, R., Induction of heat shock protein 60 expression in human monocytic cell lines infected with Mycobacterium leprae. Infection and Immunity 64(10): 4356-8 (1996, Oct). 8. Hettinga, J.V., Lemstra, W., Meijer, C., Los, G., de Vries, E.G., Konings, A.W., Kampinga, H.H., Heat-shock protein expression in cisplatin-sensitive and-resistant human tumor cells. International Journal of Cancer 67(6): 800-7 (1996, Sep 17). 9. Kligman, I., Grifo, J.A., Witkin, S.S., Expression of the 60kD heat shock protein in peritoneal fluids from women with endometriosis: implications for endometriosis-associated infertility. Human Reproduction 11(12): 2736-8 (1996). 10. Mallard, K., Jones, D.B., Richmond, J., McGill, M., Foulis, A.K., Expression of the human heat shock protein 60 in thyroid, pancreatic, hepatic and adrenal autoimmunity. Journal of Autoimmunity 9(1): 89-96 (1996).