Mouse Heat Shock Cognate Protein 70, Luminal, Endoplasmic Reticulum Monoclonal Antibody
Heat Shock Cognate Protein 70, Luminal, Endoplasmic Reticulum (HSC70, BiP)
Applications
Western Blot, Immunoprecipitation
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
Heat Shock Cognate Protein 70; Luminal; Endoplasmic Reticulum; Monoclonal Antibody; Endoplasmic Reticulum (HSC70; BiP); Anti -Heat Shock Cognate Protein 70; anti-Heat Shock Cognate Protein 70; Endoplasmic Reticulum antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
4E113
Specificity
This affinity purified antibody Detects a ~78kD protein, corresponding to the apparent molecular mass of Hsc70 plant BiP on SDS-PAGE immunoblots, in samples from apple, asparagus, carrot, corn, lettuce, orange, potato, spinach, tomato, Arabidopsis thaliana leaves, seeds and siliques, woody plant bark tissue and tobacco. Although this antibody can be used for immunoprecipitations under native conditions, it is not recommended for immunoprecipitating BiP from tomato lysates. This antibody does not cross react with E. coli DnaK, mammalian Hsc70, recombinant hamster BiP or spinach cytosolic Hsc70.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.1mM PMSF, 50% glycerol.
Applicable Applications for anti-Heat Shock Cognate Protein 70, Luminal, Endoplasmic Reticulum antibody
Western Blot (WB), Immunoprecipitation (IP)
Immunogen
A preparation of spinach Hsc70 proteins comprised of cytosolic Hsc70, BiP and possibly other members of the Hsc70 family of proteins
Preparation and Storage
-20 degree C
Related Product Information for anti-Heat Shock Cognate Protein 70, Luminal, Endoplasmic Reticulum antibody
The endoplasmic reticulum (ER) luminal Hsc70 protein, also known as BiP, is a member of the multigene 70kD family of stress proteins which is involved in the proper translocation and processing of secretory proteins passing through the ER. In spinach, BiP is expressed constitutively in all tissues, except dry seeds, and its levels remain constant in response to environmental stresses such as cold acclimation, drought stress or heat shock (2). This is in contrast to spinach BiP mRNA levels, however, which increase during cold acclimation and decrease in response to drought stress or heat shock (2). Low temperatures may alter protein biogenesis resulting in an accumulation of misfolded proteins that are chaperoned by the existing pool of free BiP protein which in turn, signals an up-regulation of BiP mRNA (2). On the other hand, drought stress and heat shock may reduce protein export through the secretory pathway, thus signaling a down-regulation of BiP mRNA (2). In any case, it appears that the level of constitutively expressed BiP protein is sufficient to maintain proper protein biogenesis when faced with environmental stresses (2).
Product Categories/Family for anti-Heat Shock Cognate Protein 70, Luminal, Endoplasmic Reticulum antibody
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Product Notes
The Heat Shock Cognate Protein 70, Luminal, Endoplasmic Reticulum (Catalog #AAA603346) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Heat Shock Cognate Protein 70, Luminal, Endoplasmic Reticulum can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the Heat Shock Cognate Protein 70, Luminal, Endoplasmic Reticulum for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Heat Shock Cognate Protein 70, Luminal, Endoplasmic Reticulum, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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