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Western Blot (WB)
(Western Blot detection against Immunogen (48.44kD).)
Mouse anti-Human HDGFRP3 Monoclonal Antibody | anti-HDGFRP3 antibody
HDGFRP3 (HDGF2, Hepatoma-derived Growth Factor-related Protein 3, HRP-3, Hepatoma-derived Growth Factor 2, HDGF-2, CGI-142) (HRP)
Gene Names
HDGFRP3; HDGF2; HRP-3; HDGF-2; CGI-142
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified
Synonyms
HDGFRP3; Monoclonal Antibody; HDGFRP3 (HDGF2; Hepatoma-derived Growth Factor-related Protein 3; HRP-3; Hepatoma-derived Growth Factor 2; HDGF-2; CGI-142) (HRP); anti-HDGFRP3 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
2G12
Specificity
Recognizes human HDGFRP3.
Purity/Purification
Purified
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Applicable Applications for anti-HDGFRP3 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Full length recombinant protein corresponding to aa1-203 from human HDGFRP3 (AAH15483) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MARPRPREYKAGDLVFAKMKGYPHWPARIDELPEGAVKPPANKYPIFFFGTHETAFLGPKDLFPYKEYKDKFGKSNKRKGFNEGLWEIENNPGVKFTGYQAIQQQSSSETEGEGGNTADASSEEEGDRVEEDGKGKRKNEKAGSKRKKSYTSKKSSKQSRKSPGDEDDKDCKEEENKSSSEGGDAGNDTRNTTSDLQKTSEGT
Conjugate
HRP
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot detection against Immunogen (48.44kD).)
Western Blot (WB)
(Western Blot detection against Immunogen (48.44kD).)
Western Blot (WB)
(Western Blot analysis of HDGFRP3 expression in transfected 293T cell line byMBS647687. Lane 1: HDGFRP3 transfected lysate (23kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of HDGFRP3 expression in transfected 293T cell line byMBS647687. Lane 1: HDGFRP3 transfected lysate (23kD). Lane 2: Non-transfected lysate.)
Immunofluorescence (IF)
(Immunofluorescence of MBS647687 (10ug/ml) on HeLa cells)
Immunofluorescence (IF)
(Immunofluorescence of MBS647687 (10ug/ml) on HeLa cells)
Testing Data
(Detection limit for recombinant GST tagged HDGFRP3 is ~0.1ng/ml as a capture antibody.)
Testing Data
(Detection limit for recombinant GST tagged HDGFRP3 is ~0.1ng/ml as a capture antibody.)
Related Product Information for anti-HDGFRP3 antibody
Enhances DNA synthesis and may play a role in cell proliferation.
Product Categories/Family for anti-HDGFRP3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
22,620 Da
NCBI Official Full Name
Homo sapiens hepatoma-derived growth factor, related protein 3, mRNA
NCBI Official Symbol
HDGFRP3
NCBI Official Synonym Symbols
HDGF2; HRP-3; HDGF-2; CGI-142
NCBI Protein Information
hepatoma-derived growth factor-related protein 3
Protein Family
Research Articles on HDGFRP3
Similar Products
Product Notes
The HDGFRP3 (Catalog #AAA6152853) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The HDGFRP3 (HDGF2, Hepatoma-derived Growth Factor-related Protein 3, HRP-3, Hepatoma-derived Growth Factor 2, HDGF-2, CGI-142) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HDGFRP3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the HDGFRP3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HDGFRP3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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