Mouse HDAC1 Monoclonal Antibody | anti-HDAC1 antibody

Mouse Monoclonal [clone 3E1] (IgG1,k) to Human HDAC1

Cat. Num. AAA246241

• Gene Names: HDAC1; HD1; RPD3; GON-10; RPD3L1
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: HDAC1; Monoclonal Antibody; Mouse Monoclonal [clone 3E1] (IgG1; k) to Human HDAC1; Anti-HDAC1 Antibody (clone 3E1) IHC-plus; Histone deacetylase 1; GON-10; HD1; RPD3; RPD3L1; Human HDAC1; anti-HDAC1 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 3E1
• Specificity: Human HDAC1
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.4.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 482

• Applicable Applications for anti-HDAC1 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IF (10 ug/ml), IHC-P (5 ug/ml), WB (1:500 - 1:1000); Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Cell lysate, Cell lysate, Cell lysate, Recombinant protein.
• Target Species: Human
• Immunogen Description: HDAC1 (AAH00301, 1 a.a. ~ 483 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen: HDAC1 antibody was raised against hDAC1 (AAH00301, 1 a.a. ~ 483 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Aliquot and store at -20 degree C or -80 degree C. Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-HDAC1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to HDAC1 on HeLa cell. [antibody concentration 10 ug/ml])

Western Blot (WB)

(Western blot of HDAC1 expression in NIH/3T3 cell lysate.)

Western Blot (WB)

(Western blot of HDAC1 expression in HeLa nuclear extract.)

ELISA (EIA)

(Detection limit for recombinant GST tagged HDAC1 is approximately 0.1 ng/ml as a capture antibody.)

Product Categories/Family for anti-HDAC1 antibody

Family: Histone Deacetylase

NCBI and Uniprot Product Information

• NCBI GI #: 13128860
• NCBI GeneID: 3065
• NCBI Accession #: NP_004955.2
• NCBI GenBank Nucleotide #: NM_004964.2
• UniProt Accession #: Q13547; Q92534
• Molecular Weight: 55,103 Da
• NCBI Official Full Name: histone deacetylase 1
• NCBI Official Synonym Full Names: histone deacetylase 1
• NCBI Official Symbol: HDAC1
• NCBI Official Synonym Symbols: HD1; RPD3; GON-10; RPD3L1
• NCBI Protein Information: histone deacetylase 1; reduced potassium dependency, yeast homolog-like 1
• UniProt Protein Name: Histone deacetylase 1
• UniProt Gene Name: HDAC1
• UniProt Synonym Gene Names: RPD3L1; HD1
• UniProt Entry Name: HDAC1_HUMAN

NCBI Description

Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq, Jul 2008]

Uniprot Description

HDAC1: a transcriptional regulator of the histone deacetylase family, subfamily 1. Deacetylates lysine residues on the N-terminal part of the core histones H2A, H2B, H3 AND H4. Plays an important role in transcriptional regulation, cell cycle progression and developmental events.

Protein type: Nuclear receptor co-regulator; Hydrolase; EC 3.5.1.98

Chromosomal Location of Human Ortholog: 1p34

Cellular Component: nucleoplasm; protein complex; Sin3 complex; cytoplasm; histone deacetylase complex; nuclear chromatin; NuRD complex; chromatin; nucleus; cytosol

Molecular Function: deacetylase activity; nucleosomal DNA binding; transcription activator binding; histone deacetylase binding; protein deacetylase activity; protein N-terminus binding; transcription factor binding; protein binding; NAD-dependent histone deacetylase activity (H3-K9 specific); enzyme binding; NAD-dependent histone deacetylase activity (H3-K14 specific); NAD-dependent histone deacetylase activity (H4-K16 specific); histone deacetylase activity; transcription factor activity

Biological Process: establishment and/or maintenance of chromatin architecture; nerve growth factor receptor signaling pathway; viral reproduction; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic; negative regulation of cell cycle; protein amino acid deacetylation; negative regulation of gene expression, epigenetic; transforming growth factor beta receptor signaling pathway; positive regulation of cell proliferation; circadian regulation of gene expression; transcription initiation from RNA polymerase II promoter; Notch signaling pathway; transcription, DNA-dependent; histone deacetylation; chromatin modification; odontogenesis of dentine-containing teeth; chromatin remodeling; ATP-dependent chromatin remodeling; epidermal cell differentiation; gene expression; positive regulation of transcription from RNA polymerase II promoter; embryonic digit morphogenesis; mitotic cell cycle; blood coagulation; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis