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Western Blot (WB) (Western blot analysis of HAPLN1 on different lysates using anti-HAPLN1 antibody at 1/1, 000 dilution. Positive control: Lane 1: BT-20 Lane 2: HUVEC Lane 3: SW480)

Rabbit HAPLN1 Monoclonal Antibody | anti-HAPLN1 antibody

HAPLN1 Antibody

Gene Names
HAPLN1; CRTL1
Reactivity
Human, Mouse, Rat, Zebrafish
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
Purity
ProA affinity purified
Synonyms
HAPLN1; Monoclonal Antibody; HAPLN1 Antibody; Cartilage link protein antibody; cartilage linking protein 1 antibody; Cartilage-link protein antibody; Cartilage-linking protein 1 antibody; CLP antibody; CRTL1 antibody; Crtl1l antibody; HAPLN1 antibody; HPLN1_HUMAN antibody; Hyaluronan and proteoglycan link protein 1 antibody; Hyaluronan and proteoglycan link protein 1 precursor antibody; LP antibody; Proteoglycan link protein antibody; anti-HAPLN1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat, Zebrafish
Clonality
Monoclonal
Clone Number
SY02-17
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-HAPLN1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes
WB: 1:1000-5000
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of HAPLN1 on different lysates using anti-HAPLN1 antibody at 1/1, 000 dilution. Positive control: Lane 1: BT-20 Lane 2: HUVEC Lane 3: SW480)

Western Blot (WB) (Western blot analysis of HAPLN1 on different lysates using anti-HAPLN1 antibody at 1/1, 000 dilution. Positive control: Lane 1: BT-20 Lane 2: HUVEC Lane 3: SW480)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HAPLN1 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HAPLN1 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-HAPLN1 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-HAPLN1 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-HAPLN1 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-HAPLN1 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-HAPLN1 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-HAPLN1 antibody. Counter stained with hematoxylin.)

Immunocytochemistry (ICC)

(ICC staining HAPLN1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC) (ICC staining HAPLN1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC)

(ICC staining HAPLN1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC) (ICC staining HAPLN1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC)

(ICC staining HAPLN1 in BT-20 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC) (ICC staining HAPLN1 in BT-20 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Related Product Information for anti-HAPLN1 antibody
The human cartilage link protein, also designated HAPLN1, is a member of the hyaluronan and proteoglycan link protein (HAPLN) family of proteins. This family contains 4 proteins with approximately 50% homology. The human cartilage link protein strengthens tissue architecture by using hyaluronic acid to stabilize the aggregates of proteoglycan monomers inside the extracellular cartilage matrix. It is widely expressed in most tissue types except brain tissue where, much like the other human cartilage link protein genes, it is weakly expressed. The HAPLN and the brain-specific CSPG genes, as well as other members of the link module superfamily, appear to have a common ancestral gene origin.
Product Categories/Family for anti-HAPLN1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
40,166 Da
NCBI Official Full Name
hyaluronan and proteoglycan link protein 1
NCBI Official Synonym Full Names
hyaluronan and proteoglycan link protein 1
NCBI Official Symbol
HAPLN1
NCBI Official Synonym Symbols
CRTL1
NCBI Protein Information
hyaluronan and proteoglycan link protein 1; Cartilage link protein; cartilage-link protein; cartilage linking protein 1; cartilage-linking protein 1
UniProt Protein Name
Hyaluronan and proteoglycan link protein 1
UniProt Gene Name
HAPLN1
UniProt Synonym Gene Names
CRTL1; Cartilage-link protein
UniProt Entry Name
HPLN1_HUMAN

Uniprot Description

HAPLN1: Stabilizes the aggregates of proteoglycan monomers with hyaluronic acid in the extracellular cartilage matrix. Belongs to the HAPLN family.

Protein type: Extracellular matrix

Chromosomal Location of Human Ortholog: 5q14.3

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular region

Molecular Function: extracellular matrix structural constituent; hyaluronic acid binding

Biological Process: extracellular matrix organization and biogenesis; central nervous system development; cell adhesion; skeletal development

Research Articles on HAPLN1

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Product Notes

The HAPLN1 hapln1 (Catalog #AAA9435623) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HAPLN1 Antibody reacts with Human, Mouse, Rat, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's HAPLN1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC). WB: 1:1000-5000. Researchers should empirically determine the suitability of the HAPLN1 hapln1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HAPLN1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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