Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant HAND1 protein (Right) or empty vector (Left) detected with HAND1 antibody)
Mouse HAND1 Monoclonal Antibody | anti-HAND1 antibody
HAND1 antibody
FC: 1:100
WB: 1:500
Upon receipt, store at -20°C. Avoid repeat freeze-thaw cycles.
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant HAND1 protein (Right) or empty vector (Left) detected with HAND1 antibody)
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant HAND1 protein (Right) or empty vector (Left) detected with HAND1 antibody)
Immunofluorescence (IF)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant HAND1 protein using HAND1 antibody)
Immunofluorescence (IF)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant HAND1 protein using HAND1 antibody)
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of Hela cells using HAND1 antibody (Red), compared to a nonspecific negative control antibody (Blue).)
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of Hela cells using HAND1 antibody (Red), compared to a nonspecific negative control antibody (Blue).)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, it has been suggested that this transcription factor may be required for early trophoblast differentiation. [provided by RefSeq, Jul 2008]
Uniprot Description
HAND1: Transcription factor that plays an essential role in both trophoblast-giant cells differentiation and in cardiac morphogenesis. In the adult, could be required for ongoing expression of cardiac-specific genes. Binds the DNA sequence 5'- NRTCTG-3' (non-canonical E-box).
Protein type: Cell development/differentiation; DNA-binding; Transcription factor; Nucleolus
Chromosomal Location of Human Ortholog: 5q33
Cellular Component: nucleoplasm; cytoplasm; nucleolus; nucleus
Molecular Function: identical protein binding; protein binding; protein homodimerization activity; enzyme binding; protein heterodimerization activity; transcription coactivator activity; bHLH transcription factor binding; transcription corepressor activity; transcription factor binding
Biological Process: transcription from RNA polymerase II promoter; blastocyst development; mesoderm formation; negative regulation of transcription factor activity; heart development; embryonic heart tube development; ventricular cardiac muscle morphogenesis; positive regulation of transcription from RNA polymerase II promoter; heart looping; negative regulation of transcription from RNA polymerase II promoter; angiogenesis; negative regulation of transcription, DNA-dependent; odontogenesis of dentine-containing teeth; trophectodermal cell differentiation
Research Articles on HAND1
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Product Notes
The HAND1 hand1 (Catalog #AAA831724) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's HAND1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB). IF: 1:100 FC: 1:100 WB: 1:500. Researchers should empirically determine the suitability of the HAND1 hand1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HAND1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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