Western Blot (WB)
(Figure 1: Western blot analysis using GYS1 mouse mAb against Hela (1) and HEK293 (2) cell lysate.)
Mouse anti-Human, Mouse GYS1 Monoclonal Antibody | anti-GYS1 antibody
GYS1 Antibody
Gene Names
GYS1; GSY; GYS
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA, Flow Cytometry, Functional Assay
Purity
Affinity-Chromatography
Synonyms
GYS1; Monoclonal Antibody; GYS1 Antibody; Glycogen [starch] synthase; Glycogen synthase 1 (muscle); Glycogen synthase 1; GSY; GYS; Gys1; GYS1_HUMAN; muscle; anti-GYS1 antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG1
Specificity
GYS1 antibody detects endogenous levels of total GYS1
Purity/Purification
Affinity-Chromatography
Form/Format
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
673
Applicable Applications for anti-GYS1 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA), Flow Cytometry (FC/FACS)
Application Notes
ELISA: 1:10000
WB: 1:500-1:2000
FC/FACS: 1:200-1:400
IF/ICC: 1:100-1:500
WB: 1:500-1:2000
FC/FACS: 1:200-1:400
IF/ICC: 1:100-1:500
Immunogen
Purified recombinant fragment of human GYS1 expressed in E. Coli
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Figure 1: Western blot analysis using GYS1 mouse mAb against Hela (1) and HEK293 (2) cell lysate.)
Western Blot (WB)
(Figure 1: Western blot analysis using GYS1 mouse mAb against Hela (1) and HEK293 (2) cell lysate.)
Related Product Information for anti-GYS1 antibody
Description: GYS1 muscle glycogen synthase 1. Transfers glucosyl residue from UDP-glucose to glycogen. Regulated allosterically by glucose-6-phosphate, and by PKA-mediated phosphorylation.
Function: Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1, 4-glucan.
Subunit Structure: Interacts with GYG1.
Post-translational Modifications: Phosphorylation at Ser-8 by AMPK inactivates the enzyme activity. Primed phosphorylation at Ser-657 (site 5) by CSNK2A1 and CSNK2A2 is required for inhibitory phosphorylation at Ser-641 (site 3a), Ser-645 (site 3b), Ser-649 (site 3c) and Ser-653 (site 4) by GSK3A an GSK3B (By similarity). Phosphorylated at Ser-641 by DYRK2, leading to inactivation (By similarity). Phosphorylated at Ser-641 by PASK, leading to inactivation; phosphorylation by PASK is inhibited by glycogen. Dephosphorylation at Ser-641 and Ser-645 by PP1 activates the enzyme.
Similarity: Belongs to the glycosyltransferase 3 family.
Function: Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1, 4-glucan.
Subunit Structure: Interacts with GYG1.
Post-translational Modifications: Phosphorylation at Ser-8 by AMPK inactivates the enzyme activity. Primed phosphorylation at Ser-657 (site 5) by CSNK2A1 and CSNK2A2 is required for inhibitory phosphorylation at Ser-641 (site 3a), Ser-645 (site 3b), Ser-649 (site 3c) and Ser-653 (site 4) by GSK3A an GSK3B (By similarity). Phosphorylated at Ser-641 by DYRK2, leading to inactivation (By similarity). Phosphorylated at Ser-641 by PASK, leading to inactivation; phosphorylation by PASK is inhibited by glycogen. Dephosphorylation at Ser-641 and Ser-645 by PP1 activates the enzyme.
Similarity: Belongs to the glycosyltransferase 3 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 85 kDa
Predicted: 84 kDa
Predicted: 84 kDa
NCBI Official Full Name
glycogen
NCBI Official Synonym Full Names
glycogen synthase 1
NCBI Official Symbol
GYS1
NCBI Official Synonym Symbols
GSY; GYS
NCBI Protein Information
glycogen [starch] synthase, muscle
UniProt Protein Name
Glycogen [starch] synthase, muscle
Protein Family
UniProt Gene Name
GYS1
UniProt Synonym Gene Names
GYS
NCBI Description
The protein encoded by this gene catalyzes the addition of glucose monomers to the growing glycogen molecule through the formation of alpha-1,4-glycoside linkages. Mutations in this gene are associated with muscle glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]
Uniprot Description
Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.
Research Articles on GYS1
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Product Notes
The GYS1 gys1 (Catalog #AAA9602666) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GYS1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GYS1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA), Flow Cytometry (FC/FACS). ELISA: 1:10000 WB: 1:500-1:2000 FC/FACS: 1:200-1:400 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the GYS1 gys1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GYS1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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