Mouse anti-Human GTF2E2 Monoclonal Antibody | anti-GTF2E2 antibody

GTF2E2 (TF2E2, Transcription Initiation Factor IIE Subunit beta, General Transcription Factor IIE Subunit 2)

Cat. Num. AAA6001545

• Reactivity: Human
• Applications: ELISA, Western Blot, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: GTF2E2; Monoclonal Antibody; GTF2E2 (TF2E2; Transcription Initiation Factor IIE Subunit beta; General Transcription Factor IIE Subunit 2); Anti -GTF2E2 (TF2E2; anti-GTF2E2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 2C3
• Specificity: Recognizes human GTF2E2.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MDPSLLRERELFKKRALSTPVVEKRSASSESSSSSSKKKKTKVEHGGSSGSKQNSDHSNGSFNLKALSGSSGYKFGVLAKIVNYMKTRHQRGDTHPLTLDEILDETQHLDIGLKQKQWLMTEALVNNPKIEVIDGKYAFKPKYNVRDKKALLRLLDQHDQRGLGGILLEDIEEALPNSQKAVKALGDQILFVNRPDKKKILFFNDKSCQFSVDEEFQKLWRSVTVDSMDEEKIEEYLKRQGISSMQESGPKKVAPIQRRKKPASQKKRRFKTHNEHLAGVLKDYSDITSSK

• Applicable Applications for anti-GTF2E2 antibody: ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence, ELISA and Western Blot.; Dilution: Immunofluorescence: 40ug/ml
• Immunogen: Full length recombinant corresponding to aa1-291 from human GTF2E2 (AAH30572) with GST tag. MW of the GST tag alone is 26kD.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (57.75kD).)

Western Blot (WB)

(GTF2E2 monoclonal antibody, Western Blot analysis of GTF2E2 expression in HeLa NE.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to GTF2E2 on HeLa cell. [antibody concentration 40ug/ml].)

Related Product Information for anti-GTF2E2 antibody

GTF2E2 recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.

Product Categories/Family for anti-GTF2E2 antibody

Antibodies; Abs to Translation Initiation Factors

NCBI and Uniprot Product Information

• NCBI GI #: 114051554
• NCBI GeneID: 510921
• NCBI Accession #: NP_001039530.1
• NCBI GenBank Nucleotide #: NM_001046065.2
• UniProt Accession #: Q2KJF9
• Molecular Weight: 32,731 Da
• NCBI Official Full Name: general transcription factor IIE subunit 2
• NCBI Official Synonym Full Names: general transcription factor IIE, polypeptide 2, beta 34kDa<
• NCBI Official Symbol: GTF2E2
• NCBI Protein Information: general transcription factor IIE subunit 2; TFIIE-beta; transcription initiation factor IIE subunit beta
• UniProt Protein Name: General transcription factor IIE subunit 2
• Protein Family: General transcription factor
• UniProt Gene Name: GTF2E2
• UniProt Synonym Gene Names: TFIIE-beta
• UniProt Entry Name: T2EB_BOVIN

Uniprot Description

Function: Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase

By similarity.

Subunit structure: Tetramer of two alpha and two beta chains. Interacts with FACT subunit SUPT16H

By similarity. Interacts with ATF7IP

By similarity.

Subcellular location: Nucleus

By similarity.

Sequence similarities: Belongs to the TFIIE beta subunit family.Contains 1 TFIIE beta DNA-binding domain.