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Western Blot (WB) (Western Blot detection against Immunogen (36.74kD).)

Mouse anti-Human, Mouse GSTM2 Monoclonal Antibody | anti-GSTM2 antibody

GSTM2 (Glutathione S-transferase Mu 2, GST Class-mu 2, GSTM2-2, GST4, MGC117303) (Biotin)

Gene Names
GSTM2; GST4; GSTM; GTHMUS; GSTM2-2
Reactivity
Human, Mouse
Applications
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
GSTM2; Monoclonal Antibody; GSTM2 (Glutathione S-transferase Mu 2; GST Class-mu 2; GSTM2-2; GST4; MGC117303) (Biotin); anti-GSTM2 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
1E10
Specificity
Recognizes human GSTM2. Species Crossreactivity: mouse.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Applicable Applications for anti-GSTM2 antibody
ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
IHC-P: 3ug/ml
IF: 10ug/ml
Applications are based on unconjugated antibody.
Immunogen
Partial recombinant corresponding to aa90-189 from human GSTM2 (NP_000839) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
SEKEQIREDILENQFMDSRMQLAKLCYDPDFEKLKPEYLQALPEMLKLYSQFLGKQPWFLGDKITFVDFIAYDVLERNQVFEPSCLDAFPNLKDFISRFE
Conjugate
Biotin
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (36.74kD).)

Western Blot (WB) (Western Blot detection against Immunogen (36.74kD).)

Western Blot (WB)

(GSTM2 monoclonal antibody. Western Blot analysis of GSTM2 expression in NIH/3T3.)

Western Blot (WB) (GSTM2 monoclonal antibody. Western Blot analysis of GSTM2 expression in NIH/3T3.)

Immunohistochemistry (IHC)

(Immunoperoxidase of monoclonal antibody to GSTM2 on formalin-fixed paraffin-embedded human breast cancer. [antibody concentration 3ug/ml].)

Immunohistochemistry (IHC) (Immunoperoxidase of monoclonal antibody to GSTM2 on formalin-fixed paraffin-embedded human breast cancer. [antibody concentration 3ug/ml].)

Testing Data

(Detection limit for recombinant GST tagged GSTM2 is 0.03ng/ml as a capture antibody.)

Testing Data (Detection limit for recombinant GST tagged GSTM2 is 0.03ng/ml as a capture antibody.)
Product Categories/Family for anti-GSTM2 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
27.9 kDa (238aa), confirmed by MALDI-TOF
NCBI Official Full Name
glutathione S-transferase Mu 2 isoform 1
NCBI Official Synonym Full Names
glutathione S-transferase mu 2
NCBI Official Symbol
GSTM2
NCBI Official Synonym Symbols
GST4; GSTM; GTHMUS; GSTM2-2
NCBI Protein Information
glutathione S-transferase Mu 2
UniProt Protein Name
Glutathione S-transferase Mu 2
Protein Family
UniProt Gene Name
GSTM2
UniProt Synonym Gene Names
GST4
UniProt Entry Name
GSTM2_HUMAN

NCBI Description

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. [provided by RefSeq, Jul 2008]

Uniprot Description

GSTM2: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Belongs to the GST superfamily. Mu family.

Protein type: Transferase; Motility/polarity/chemotaxis; EC 2.5.1.18

Chromosomal Location of Human Ortholog: 1p13.3

Cellular Component: sarcoplasmic reticulum; cytoplasm; cytosol

Molecular Function: protein homodimerization activity; enzyme binding; glutathione transferase activity; glutathione binding; receptor binding

Biological Process: regulation of skeletal muscle contraction via regulation of the release of sequestered calcium ion; glutathione metabolic process; nitrobenzene metabolic process; xenobiotic metabolic process; xenobiotic catabolic process

Research Articles on GSTM2

Similar Products

Product Notes

The GSTM2 gstm2 (Catalog #AAA6142186) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GSTM2 (Glutathione S-transferase Mu 2, GST Class-mu 2, GSTM2-2, GST4, MGC117303) (Biotin) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GSTM2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IHC-P: 3ug/ml IF: 10ug/ml Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the GSTM2 gstm2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GSTM2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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