Mouse GSK3beta Monoclonal Antibody | anti-GSK3beta antibody

GSK3beta Mouse Monoclonal Antibody (4D2)

Cat. Num. AAA9700517

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
• Synonyms: GSK3beta; Monoclonal Antibody; GSK3beta Mouse Monoclonal Antibody (4D2); Mouse Anti-GSK3beta Mouse Monoclonal Antibody (4D2); GSK3B; anti-GSK3beta antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: GSK3beta protein detects endogenous levels of GSK3B.
• Purity/Purification: Affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
• Form/Format: Liquid; PBS Containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 420

• Applicable Applications for anti-GSK3beta antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: WB: 1:1000-1:2000; IHC-P: 1:100-1:200
• Immunogen: Synthetic Peptide of GSK3beta at AA range of 1-80
• Preparation and Storage: Stable for one year at -20 degree C from date of shipment.; For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Fig.1. Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using GSK3beta Mouse mAb diluted at 1:200.)

Immunohistochemistry (IHC)

(Fig.2. Immunohistochemical analysis of paraffin-embedded Human Stomach Carcinoma Tissue using GSK3beta Mouse mAb diluted at 1:200.)

Western Blot (WB)

(Fig.3. Western blot analysis of 1) Hela Cell Lysate, 2) 3T3 Cell Lysate, 3) Rat Brain Tissue Lysate using GSK3beta Mouse mAb diluted at 1:1000.)

Related Product Information for anti-GSK3beta antibody

The protein encoded by GSK3B (glycogen synthase kinase 3 beta) is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in GSK3B have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of GSK3B may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for GSK3B.

NCBI and Uniprot Product Information

• NCBI GI #: 225903437
• NCBI GeneID: 2932
• NCBI Accession #: NP_001139628.1
• NCBI GenBank Nucleotide #: NP_001139628.1
• UniProt Accession #: P49841
• NCBI Official Full Name: glycogen synthase kinase-3 beta isoform 2
• NCBI Official Synonym Full Names: glycogen synthase kinase 3 beta
• NCBI Official Symbol: GSK3B
• NCBI Protein Information: glycogen synthase kinase-3 beta
• UniProt Protein Name: Glycogen synthase kinase-3 beta
• UniProt Gene Name: GSK3B
• UniProt Synonym Gene Names: GSK-3 beta
• UniProt Entry Name: GSK3B_HUMAN

NCBI Description

The protein encoded by this gene is a serine-threonine kinase belonging to the glycogen synthase kinase subfamily. It is a negative regulator of glucose homeostasis and is involved in energy metabolism, inflammation, ER-stress, mitochondrial dysfunction, and apoptotic pathways. Defects in this gene have been associated with Parkinson disease and Alzheimer disease. [provided by RefSeq, Aug 2017]

Uniprot Description

GSK3B: a proline-directed protein kinase of the GSK family. Phosphorylates and inactivates glycogen synthase. Participates in the Wnt signaling pathway. Involved in energy metabolism, neuronal cell development, and body pattern formation

Protein type: Protein kinase, CMGC; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; EC 2.7.11.26; CMGC group; GSK family; GSK subfamily

Chromosomal Location of Human Ortholog: 3q13.3

Cellular Component: centrosome; dendritic spine; cytosol; beta-catenin destruction complex; ribonucleoprotein complex; lipid raft; nucleoplasm; growth cone; cell soma; perinuclear region of cytoplasm; cytoplasm; plasma membrane; dendritic shaft; nucleus

Molecular Function: p53 binding; beta-catenin binding; protein kinase binding; integrin binding; ionotropic glutamate receptor binding; protein serine/threonine kinase activity; NF-kappaB binding; protein binding; ubiquitin protein ligase binding; tau-protein kinase activity; kinase activity; tau protein binding; ATP binding

Biological Process: circadian rhythm; glycogen metabolic process; fat cell differentiation; axon guidance; negative regulation of MAP kinase activity; genetic imprinting; re-entry into mitotic cell cycle; nerve growth factor receptor signaling pathway; positive regulation of protein binding; protein amino acid autophosphorylation; response to lithium ion; positive regulation of axon extension; Wnt receptor signaling pathway through beta-catenin; protein amino acid phosphorylation; positive regulation of protein export from nucleus; protein export from nucleus; negative regulation of protein binding; positive regulation of cell-matrix adhesion; ER overload response; myoblast fusion; negative regulation of glycogen biosynthetic process; epidermal growth factor receptor signaling pathway; response to drug; regulation of microtubule-based process; cell migration; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; hippocampus development; negative regulation of dendrite morphogenesis; negative regulation of NFAT protein import into nucleus; positive regulation of peptidyl-serine phosphorylation; organ morphogenesis; negative regulation of neuron maturation; peptidyl-serine phosphorylation; positive regulation of protein complex assembly; establishment of cell polarity; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein catabolic process; epithelial to mesenchymal transition; innate immune response; positive regulation of transcription from RNA polymerase II promoter; negative regulation of protein complex assembly; regulation of neuronal synaptic plasticity; negative regulation of apoptosis