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Western Blot (WB) (Western blot analysis of porcine kidney ESK-4 cell lysate, probed with MBS600659.)

Mouse GS28 Monoclonal Antibody | anti-GOSR1 antibody

GS28 (GOS28, p28)

Gene Names
GOSR1; P28; GS28; GOS28; GOLIM2; GOS-28; GOS28/P28
Reactivity
Bovine, Canine, Chicken, Hamster, Mouse, Porcine, Rabbit, Rat, Sheep
Applications
Western Blot
Purity
Affinity Purified
Purified by Protein G affinity chromatography.
Synonyms
GS28; Monoclonal Antibody; GS28 (GOS28; p28); Anti -GS28 (GOS28; anti-GOSR1 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Bovine, Canine, Chicken, Hamster, Mouse, Porcine, Rabbit, Rat, Sheep
Clonality
Monoclonal
Isotype
IgG1
Clone Number
4E99
Specificity
Recognizes rat GS28 at~28kD. Recognized as a Cis Golgi marker, exposed to the cytosol, as determined by indirect immunofluoresence microscopy. Species Crossreactivity: mouse, hamster, rabbit, bovine, sheep, porcine, canine and chicken.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% azide, 50% glycerol.
Applicable Applications for anti-GOSR1 antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot (ECL): 1:1000
Immunogen
Rat liver Golgi-enriched membranes.
Positive Control
Rat Brain Tissue Extract
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of porcine kidney ESK-4 cell lysate, probed with MBS600659.)

Western Blot (WB) (Western blot analysis of porcine kidney ESK-4 cell lysate, probed with MBS600659.)
Related Product Information for anti-GOSR1 antibody
GS28, also known as p28 or GOS28, is a 28kD integral membrane protein on the surface of the Golgi apparatus that serves as a t-SNARE in ER to Golgi transport (2). The N-terminal of GS28/GOS28 is exposed to the cytosol and is anchored to the cis Golgi via a 20 amino acid carboxyl-terminal hydrophobic tail (2). GS28 co-immunoprecipitates complexes consisting of syntaxin 5, rbet1, membrin, rsec22, and rsly1, and is therefore implicated in ER-to-Golgi or intra-Golgi vesicle transport (3). ATP hydrolysis by NSF in the presence of alpha-SNAP causes the dissociation of GOS28 and syntaxin 5 complex (3).
Product Categories/Family for anti-GOSR1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
28,613 Da
NCBI Official Full Name
Golgi SNAP receptor complex member 1 isoform 2
NCBI Official Synonym Full Names
golgi SNAP receptor complex member 1
NCBI Official Symbol
GOSR1
NCBI Official Synonym Symbols
P28; GS28; GOS28; GOLIM2; GOS-28; GOS28/P28
NCBI Protein Information
Golgi SNAP receptor complex member 1; cis-golgi SNARE; Golgi SNARE 28 kDa; 28 kDa Golgi SNARE protein; 28 kDa cis-Golgi SNARE p28; golgi integral membrane protein 2
UniProt Protein Name
Golgi SNAP receptor complex member 1
UniProt Gene Name
GOSR1
UniProt Synonym Gene Names
GS28; GOS-28
UniProt Entry Name
GOSR1_HUMAN

NCBI Description

This gene encodes a trafficking membrane protein which transports proteins among the endoplasmic reticulum and the Golgi and between Golgi compartments. This protein is considered an essential component of the Golgi SNAP receptor (SNARE) complex. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

GOSR1: a single-pass type IV Golgi membrane protein. Enriched on vesicular components at the terminal rims of the Golgi. Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport. Identified in a unique SNARE complex composed of the Golgi SNAREs GOSR2, STX5, GABARAPL2 and YKT6.

Protein type: Vesicle; Autophagy; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17q11

Cellular Component: SNARE complex; Golgi membrane; Golgi apparatus; cis-Golgi network; membrane; integral to membrane

Molecular Function: SNAP receptor activity

Biological Process: intra-Golgi vesicle-mediated transport; protein transport; ER to Golgi vesicle-mediated transport; retrograde transport, endosome to Golgi

Research Articles on GOSR1

Similar Products

Product Notes

The GOSR1 gosr1 (Catalog #AAA600659) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GS28 (GOS28, p28) reacts with Bovine, Canine, Chicken, Hamster, Mouse, Porcine, Rabbit, Rat, Sheep and may cross-react with other species as described in the data sheet. AAA Biotech's GS28 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot (ECL): 1:1000. Researchers should empirically determine the suitability of the GOSR1 gosr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GS28, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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