Mouse anti-Human GRP170 Monoclonal Antibody | anti-GRP170 antibody

Mouse Anti-Human GRP170 Monoclonal IgG2aK

Cat. Num. AAA807116

• Gene Names: HYOU1; Grp170; HSP12A; ORP150; GRP-170; ORP-150
• Reactivity: Human
• Applications: Western Blot
• Purity: Protein G Purified
• Synonyms: GRP170; Monoclonal Antibody; Mouse Anti-Human GRP170 Monoclonal IgG2aK; ORP150 Antibody; HSP12A Antibody; Hypoxia up regulated 1 Antibody; Orp150 Antibody; Glucose regulated Antibody 170 Antibody; 150kDa oxygen regulated Antibody Antibody; GRP170 Antibody: ATTO 488; Mouse Anti-Human GRP170 Monoclonal; Clone 6G7-2H5; Anti-GRP170; GRP170 Antibody; anti-GRP170 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 6G7-2H5
• Purity/Purification: Protein G Purified
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 559

• Applicable Applications for anti-GRP170 antibody: Western Blot (WB)
• Application Notes: Western Blot: 1:1000
• Immunogen: Raised against a synthetic peptide of human GRP170
• Immunogen Species: Human
• COA: 1 ug/ml was sufficient for detection of GRP170 in 20 ug of HEK293 lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.1% sodium azide
• Conjugate: ATTO 488
• Cellular Localization: Endoplasmic Reticulum, Endoplasmic Reticulum Lumen
• Preparation and Storage: Store at -20 degree C

Immunocytochemistry (ICC)

(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GRP170 Monoclonal Antibody, Clone 6G7-2H5 . Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GRP170 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Endoplasmic Reticulum. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GRP170 Antibody. (D) Composite.)

Western Blot (WB)

(Western Blot analysis of Human HEK-293 cell lysate showing detection of ~170 kDa GRP170 protein using Mouse Anti-GRP170 Monoclonal Antibody, Clone 6G7-2H5 . Lane 1: Molecular Weight Ladder (MW). Lane 2: HEK-293 cell lysate. Load: 20 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-GRP170 Monoclonal Antibody at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:100 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~170 kDa.)

Related Product Information for anti-GRP170 antibody

GRP170, also known as ORP150, is the largest member of glucose-regulated Antibodys, and acts as a human chaperone Antibody. It is thought to play an important role in Antibody folding and secretion in the ER. Suppression of the Antibody is associated with accelerated apoptosis, therefore having an important cryoprotective role in hypoxia-induced cellular pertubation. This cryopotective role has led to an anti-tumor immune response, which will hopefully lead to therapeutic immunizations against cancers (1). GRP170 has also been shown to bind with dendritic cells and provide the danger signals to induce anti-tumor immune responses (2).

Product Categories/Family for anti-GRP170 antibody

Cancer; Heat Shock; Neuroscience; Neurodegeneration; Epigenetics; Trafficking; cardiovascular System

References

1. Wang H., et al. (2014) Front Oncol. 4: 377. 2. Manjili M.H., et al. (2006) Immun. Cell Biol. 84: 203-208.

NCBI and Uniprot Product Information

• NCBI GeneID: 10525
• UniProt Accession #: Q9Y4L1; Q2I204; Q53H25; A8C1Z0; B7Z909
• Molecular Weight: Detects ~170kDa.
• NCBI Official Synonym Full Names: hypoxia up-regulated 1
• NCBI Official Symbol: HYOU1
• NCBI Official Synonym Symbols: Grp170; HSP12A; ORP150; GRP-170; ORP-150
• NCBI Protein Information: hypoxia up-regulated protein 1
• UniProt Protein Name: Hypoxia up-regulated protein 1
• UniProt Gene Name: HYOU1
• UniProt Synonym Gene Names: GRP170; ORP150; ORP-150; GRP-170
• UniProt Entry Name: HYOU1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the heat shock protein 70 family. This gene uses alternative transcription start sites. A cis-acting segment found in the 5' UTR is involved in stress-dependent induction, resulting in the accumulation of this protein in the endoplasmic reticulum (ER) under hypoxic conditions. The protein encoded by this gene is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. This gene also has an alternative translation initiation site, resulting in a protein that lacks the N-terminal signal peptide. This signal peptide-lacking protein, which is only 3 amino acids shorter than the mature protein in the ER, is thought to have a housekeeping function in the cytosol. In rat, this protein localizes to both the ER by a carboxy-terminal peptide sequence and to mitochondria by an amino-terminal targeting signal. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]

Uniprot Description

HYOU1: Has a pivotal role in cytoprotective cellular mechanisms triggered by oxygen deprivation. May play a role as a molecular chaperone and participate in protein folding. Belongs to the heat shock protein 70 family.

Protein type: Chaperone; Heat shock protein

Chromosomal Location of Human Ortholog: 11q23.1-q23.3

Cellular Component: endoplasmic reticulum; endoplasmic reticulum lumen; extracellular region; focal adhesion; membrane; smooth endoplasmic reticulum

Molecular Function: ATP binding; chaperone binding

Biological Process: cellular protein metabolic process; receptor-mediated endocytosis; unfolded protein response