MDH1 active protein

MDH1, 1-334aa, Human, His tag, E Coli (Bioactivity Validated)

Cat. Num. AAA203418

• Gene Names: MDH1; MDHA; MOR2; MDH-s; HEL-S-32; MGC:1375
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: MDH1; 1-334aa; Human; His tag; E Coli (Bioactivity Validated); Malate dehydrogenase cytoplasmic; MDH-s; MDHA; MOR2; Malate dehydrogenase; cytoplasmic; MDH1 active protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MSEPIRVLVT GAAGQIAYSL LYSIGNGSVF GKDQPIILVL LDITPMMGVL DGVLMELQDC ALPLLKDVIATDKEDVAFKD LDVAILVGSM PRREGMERKD LLKANVKIFK SQGAALDKYA KKSVKVIVVG NPANTNCLTA SKSAPSIPKE NFSCLTRLDH NRAKAQIALK LGVTANDVKN VIIWGNHSST QYPDVNHAKV KLQGKEVGVY EALKDDSWLK GEFVTTVQQR GAAVIKARKL SSAMSAAKAI CDHVRDIWFG TPEGEFVSMG VISDGNSYGV PDDLLYSFPV VIKNKTWKFV EGLPINDFSR EKMDLTAKEL TEEKESAFEF LSSALEHHHH HH
• Sequence Length: 334

• Applicable Applications for MDH1 active protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Biological Activity: Specific activity is > 250 units/mg, and is defined as the amount of enzyme that cleaves 1umole of oxalacetate and beta-NADH to L-malate and beta-NAD per minute at pH8.0 at 37C.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for MDH1 active protein

MDH1 is one of the two malate dehydrogenases. Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. In particular, MDH1 is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. This protein also regulates p53-dependent cell-cycle arrest and apoptosis in response to glucose deprivation. Recombinant human MDH1 protein, fused to His-tag at C-terminus, was expressed in E Coli and purified by using conventional chromatography.

Product Categories/Family for MDH1 active protein

Cancer

References

Youn HD., et al. (2009) Cell Death Differ. 16 (5):738-48; Waye MM., et al (2005) J Cell Biochem. 94 (4):763-73.;

NCBI and Uniprot Product Information

• NCBI GI #: 5174539
• NCBI GeneID: 4190
• NCBI Accession #: NP_005908
• NCBI GenBank Nucleotide #: NM_005917.3
• UniProt Accession #: P40925; Q6I9V0; B2R5V5; B4DUN2; B7Z3I7; F5H098
• Molecular Weight: 37.4 kDa (342aa), confirmed by MALDI-TOF
• NCBI Official Full Name: malate dehydrogenase, cytoplasmic isoform MDH1
• NCBI Official Synonym Full Names: malate dehydrogenase 1
• NCBI Official Symbol: MDH1
• NCBI Official Synonym Symbols: MDHA; MOR2; MDH-s; HEL-S-32; MGC:1375
• NCBI Protein Information: malate dehydrogenase, cytoplasmic; malate dehydrogenase, peroxisomal
• UniProt Protein Name: Malate dehydrogenase, cytoplasmic
• Protein Family: Malate dehydrogenase
• UniProt Gene Name: MDH1
• UniProt Synonym Gene Names: MDHA

NCBI Description

This gene encodes an enzyme that catalyzes the NAD/NADH-dependent, reversible oxidation of malate to oxaloacetate in many metabolic pathways, including the citric acid cycle. Two main isozymes are known to exist in eukaryotic cells: one is found in the mitochondrial matrix and the other in the cytoplasm. This gene encodes the cytosolic isozyme, which plays a key role in the malate-aspartate shuttle that allows malate to pass through the mitochondrial membrane to be transformed into oxaloacetate for further cellular processes. Alternatively spliced transcript variants have been found for this gene. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Pseudogenes have been identified on chromosomes X and 6. [provided by RefSeq, Feb 2016]