Western Blot (WB)
(Western blot of rat PC12 cell lysate. The blot was probed with mouse monoclonal anti-Grb2 antibody at 1:250 (lane 1), 1:500 (lane 2), or 1:1000 (lane 3).)
Mouse Grb2 Monoclonal Antibody | anti-Grb2 antibody
Grb2 Antibody
Reactivity
Human, Rat, Mouse
Applications
Western Blot, ELISA, Immunoprecipitation, Immunocytochemistry
Synonyms
Grb2; Monoclonal Antibody; Grb2 Antibody; anti-Grb2 antibody
Host
Mouse
Reactivity
Human, Rat, Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
M331
Specificity
This antibody detects a 24kDa* protein corresponding to the molecular mass of Grb2 on SDS-PAGE immunoblots of rat PC12.
Form/Format
Mouse monoclonal, protein A purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-Grb2 antibody
Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), Immunocytochemistry (ICC)
Application Notes
ELISA: 1:2000
ICC: 1:50
IP: 1:50
WB: 1:1000
ICC: 1:50
IP: 1:50
WB: 1:1000
Immunogen
Clone (M331) was generated from a full length recombinant rat Grb2 protein. This sequence has high homology to human and mouse Grb2.
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
Western Blot (WB)
(Western blot of rat PC12 cell lysate. The blot was probed with mouse monoclonal anti-Grb2 antibody at 1:250 (lane 1), 1:500 (lane 2), or 1:1000 (lane 3).)
Western Blot (WB)
(Western blot of rat PC12 cell lysate. The blot was probed with mouse monoclonal anti-Grb2 antibody at 1:250 (lane 1), 1:500 (lane 2), or 1:1000 (lane 3).)
Immunocytochemistry (ICC)
(Immunocytochemical labeling of Grb2 in rat PC12 cells differentiated with NGF. The cells were labeled with mouse monoclonal Grb2 (GM3311) antibody, then detected using appropriate secondary antibody conjugated to Cy3.)
Immunocytochemistry (ICC)
(Immunocytochemical labeling of Grb2 in rat PC12 cells differentiated with NGF. The cells were labeled with mouse monoclonal Grb2 (GM3311) antibody, then detected using appropriate secondary antibody conjugated to Cy3.)
Related Product Information for anti-Grb2 antibody
Crk and Grb2 family adaptor proteins are involved in a variety of cell signaling pathways related to human diseases. These adaptors have Src homology 2 (SH2) and Src homology 3 (SH3) domains, which are docking sites for several signaling proteins, including receptors, kinases, and GTPase regulators. In addItion, other protein-protein interactions may also utilize Crk and Grb2 domain interactions to modulate cell signaling pathways. The DOCK family of proteins (DOCK3, DOCK4, and DOCK5) can interact with Crk and may be important Crk effector proteins. The cell cycle regulator p27Kip1 can interact with Grb2, and this implicates Grb2 activity in cell signaling pathways that alter cell cycle progression.
References
Feller, S.M. & Lewitzky, M. (2006) Curr. Pharm. Des. 12(5):529.
Feller, S.M. (2001) Oncogene. 20(44):6348.
Feller, S.M. (2001) Oncogene. 20(44):6348.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,206 Da
NCBI Official Full Name
growth factor receptor-bound protein 2
NCBI Official Synonym Full Names
growth factor receptor bound protein 2
NCBI Official Symbol
Grb2
NCBI Protein Information
growth factor receptor-bound protein 2; protein Ash; SH2/SH3 adapter GRB2; adapter protein GRB2
UniProt Protein Name
Growth factor receptor-bound protein 2
Protein Family
UniProt Gene Name
Grb2
UniProt Synonym Gene Names
Ash
UniProt Entry Name
GRB2_RAT
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Product Notes
The Grb2 grb2 (Catalog #AAA474371) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Grb2 Antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Grb2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), Immunocytochemistry (ICC). ELISA: 1:2000 ICC: 1:50 IP: 1:50 WB: 1:1000. Researchers should empirically determine the suitability of the Grb2 grb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Grb2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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