Mouse anti-Human GRAP2 Monoclonal Antibody | anti-GRAP2 antibody

Anti-GRAP2 PE

Cat. Num. AAA1801924

• Gene Names: GRAP2; P38; GADS; GRID; GRPL; GrbX; Mona; GRB2L; GRBLG; Grf40; GRAP-2
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: GRAP2; Monoclonal Antibody; Anti-GRAP2 PE; GADS; GRID; GRAP-2; GrbX; GRB2 related adaptor protein 2; anti-GRAP2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Clone Number: UW40
• Specificity: The mouse monoclonal antibody UW40 recognizes GRAP2/GADS, a 41kDa cytoplasmic adaptor protein that plays a pivotal role during the early events of signal transduction in T cells.
• Purity/Purification: The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
• Concentration: 0.1mg/ml (varies by lot)

• Applicable Applications for anti-GRAP2 antibody: Flow Cytometry (FC/FACS) (QC tested)
• Application Notes: FC/FACS: Recommended dilution: 1-4ug/ml. Intracellular staining.
• Conjugation: PE
• Immunogen: GST-fusion human GRAP2/GADS protein
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Flow Cytometry (FC/FACS)

(Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-human GRAP2(UW40) PE antibody (concentration in sample 1,7 ug/ml).)

Flow Cytometry (FC/FACS)

(Separation of lymphocytes stained using anti-human GRAP2 (UW40) PE antibody (concentration in sample 1,7 ug/ml, red-filled) from lymphocytes stained using mouse IgG2a isotype control (MOPC-173) PE antibody (concentration in sample 1,7 ug/ml, same as GRAP2 PE concentration, black-dashed) in flow cytometry analysis (intracellular staining) of peripheral blood.)

Related Product Information for anti-GRAP2 antibody

GRAP2/GADS (Grb2-related adaptor protein 2/Grb2-related adaptor downstream of Shc) is a cytoplasmic adaptor protein containing N- and C-terminal SH3 domains flanking a central SH2 domain and a proline/glutamine-rich region. It is expressed predominantly in lymphoid tissue and hematopoietic cells, particularly in T cells. GRAP2/GADS plays a pivotal role during the early events of T cell signal transduction by recruiting the adaptor protein SLP-76 and its associated molecules, such as Vav, Nck, Itk, and ADAP, to the transmembrane adaptor protein LAT. GRAP2/GADS also binds several other signaling proteins, namely Gab2, HPK1 (hematopoietic progenitor kinase 1), and Cbl. Unlike similar adaptor protein Grb2, GRAP2/GADS shows higher selectivity when binding to the particular phosphorylated tyrosines of LAT adaptor.

References

*Yankee TM, Yun TJ, Draves KE, Ganesh K, Bevan MJ, Murali-Krishna K, Clark EA: The Gads (GrpL) adaptor protein regulates T cell homeostasis. J Immunol. 2004 Aug 1;173(3):1711-20., URL: http://www.ncbi.nlm.nih.gov/pubmed/15265900,*Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F: A high-throughput pipeline for validation of antibodies. Nat Methods. 2018 Nov;15(11):909-912., URL: https://www.ncbi.nlm.nih.gov/pubmed/30377371,*Yankee TM, Draves KE, Ewings MK, Clark EA, Graves JD: CD95/Fas induces cleavage of the GrpL/Gads adaptor and desensitization of antigen receptor signaling. Proc Natl Acad Sci U S A. 2001 Jun 5;98(12):6789-93., URL: http://www.ncbi.nlm.nih.gov/pubmed/11391000
*Watanabe R, Harada Y, Takeda K, Takahashi J, Ohnuki K, Ogawa S, Ohgai D, Kaibara N, Koiwai O, Tanabe K, Toma H, Sugamura K, Abe R: Grb2 and Gads exhibit different interactions with CD28 and play distinct roles in CD28-mediated costimulation. J Immunol. 2006 Jul 15;177(2):1085-91., URL: https://www.ncbi.nlm.nih.gov/pubmed/16818765,*Moran O, Roessle MW, Mariuzza RA, Dimasi N: Structural features of the full-length adaptor protein GADS in solution determined using small-angle X-ray scattering. Biophys J. 2008 Mar 1;94(5):1766-72., URL: https://www.ncbi.nlm.nih.gov/pubmed/17993503,*Dalheimer SL, Zeng L, Draves KE, Hassaballa A, Jiwa NN, Parrish TD, Clark EA, Yankee TM: Gads-deficient thymocytes are blocked at the transitional single positive CD4+ stage. Eur J Immunol. 2009 May;39(5):1395-404., URL: https://www.ncbi.nlm.nih.gov/pubmed/19337995,*Cho S, Velikovsky CA, Swaminathan CP, Houtman JC, Samelson LE, Mariuzza RA: Structural basis for differential recognition of tyrosine-phosphorylated sites in the linker for activation of T cells (LAT) by the adaptor Gads. EMBO J. 2004 Apr 7;23(7):1441-51, URL: https://www.ncbi.nlm.nih.gov/pubmed/15029250

NCBI and Uniprot Product Information

• NCBI GI #: 4758476
• NCBI GeneID: 9402
• NCBI Accession #: NP_004801.1
• NCBI GenBank Nucleotide #: NM_004810.2
• UniProt Accession #: O75791; O43726; Q9NRB7
• Molecular Weight: 37,909 Da
• NCBI Official Full Name: GRB2-related adapter protein 2
• NCBI Official Synonym Full Names: GRB2-related adaptor protein 2
• NCBI Official Symbol: GRAP2
• NCBI Official Synonym Symbols: P38; GADS; GRID; GRPL; GrbX; Mona; GRB2L; GRBLG; Grf40; GRAP-2
• NCBI Protein Information: GRB2-related adapter protein 2; grf-40; GRB-2-like protein; adapter protein GRID; grf40 adapter protein; SH3-SH2-SH3 adapter Mona; SH3-SH2-SH3 adaptor molecule; growth factor receptor-binding protein; GRB2-related protein with insert domain; hematopoietic
• UniProt Protein Name: GRB2-related adapter protein 2
• Protein Family: GRB2-related adapter protein
• UniProt Gene Name: GRAP2
• UniProt Synonym Gene Names: GADS; GRB2L; GRID; GRB2L; Grf-40
• UniProt Entry Name: GRAP2_HUMAN

NCBI Description

This gene encodes a member of the GRB2/Sem5/Drk family. This member is an adaptor-like protein involved in leukocyte-specific protein-tyrosine kinase signaling. Like its related family member, GRB2-related adaptor protein (GRAP), this protein contains an SH2 domain flanked by two SH3 domains. This protein interacts with other proteins, such as GRB2-associated binding protein 1 (GAB1) and the SLP-76 leukocyte protein (LCP2), through its SH3 domains. Transcript variants utilizing alternative polyA sites exist. [provided by RefSeq, Jul 2008]

Uniprot Description

GADS: Interacts with SLP-76 to regulate NF-AT activation. Binds to tyrosine-phosphorylated shc. Belongs to the GRB2/sem-5/DRK family.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 22q13.2

Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol; endosome

Molecular Function: protein binding; SH3/SH2 adaptor activity

Biological Process: positive regulation of signal transduction; cell-cell signaling; Ras protein signal transduction; T cell costimulation; innate immune response; T cell receptor signaling pathway