Testing Data
(Sandwich ELISA analysis of human granzyme B using Mouse anti Human granzyme B as a capture reagent and biotinylated Mouse anti Human granzyme B (MBS210398) as a detection reagent with recombinant human granzyme B as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . Human plasma samples are diluted i:2 (green) and 1:4 (blue))
Mouse GRANZYME B Monoclonal Antibody | anti-GZMB antibody
MOUSE ANTI GRANZYME B
Purified IgG - liquid
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
ELISA: Minimum Dilution: 2ug/ml; Maximum Dilution: 5ug/ml
Flow Cytometry: Minimum Dilution: 1/10; Maximum Dilution: 1/100; Application Note: Membrane permeabilisation is required for this application. MyBioSource recommends the use of Leucoperm for this purpose.
Preparation
Shelf Life: 18 months from date of despatch.
Testing Data
(Sandwich ELISA analysis of human granzyme B using Mouse anti Human granzyme B as a capture reagent and biotinylated Mouse anti Human granzyme B (MBS210398) as a detection reagent with recombinant human granzyme B as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . Human plasma samples are diluted i:2 (green) and 1:4 (blue))
Testing Data
(Sandwich ELISA analysis of human granzyme B using Mouse anti Human granzyme B as a capture reagent and biotinylated Mouse anti Human granzyme B (MBS210398) as a detection reagent with recombinant human granzyme B as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . Human plasma samples are diluted i:2 (green) and 1:4 (blue))
NCBI and Uniprot Product Information
NCBI Description
Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein encoded by this gene is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response. [provided by RefSeq, Jul 2008]
Uniprot Description
GZMB: This enzyme is necessary for target cell lysis in cell- mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. By staphylococcal enterotoxin A (SEA) in peripheral blood leukocytes. Inactivated by the serine protease inhibitor diisopropylfluorophosphate. Belongs to the peptidase S1 family. Granzyme subfamily.
Protein type: EC 3.4.21.79; Apoptosis; Protease
Chromosomal Location of Human Ortholog: 14q11.2
Cellular Component: membrane; intracellular membrane-bound organelle; cytoplasm; immunological synapse; nucleus; cytosol
Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity
Biological Process: Notch signaling pathway; induction of apoptosis by granzyme; apoptosis; natural killer cell mediated cytotoxicity; cytolysis; immune response; protein processing; proteolysis
Research Articles on GZMB
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Product Notes
The GZMB gzmb (Catalog #AAA212507) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's GRANZYME B can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, ELISA (EIA), Flow cytometry (FC/FACS)*, Immunofluorescence (IF), Immunoprecipitation (IP). ELISA: This antibody has been reported to function as a capture reagent in sandwich ELISA assays for soluble Granzyme B in conjunction with biotin conjugated clone GB10 as detection reagent. Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood. ELISA: Minimum Dilution: 2ug/ml; Maximum Dilution: 5ug/ml Flow Cytometry: Minimum Dilution: 1/10; Maximum Dilution: 1/100; Application Note: Membrane permeabilisation is required for this application. MyBioSource recommends the use of Leucoperm for this purpose. Researchers should empirically determine the suitability of the GZMB gzmb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GRANZYME B, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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