Mouse GRANZYME A Monoclonal Antibody | anti-GZMA antibody

MOUSE ANTI HUMAN GRANZYME A

Cat. Num. AAA212504

• Gene Names: GZMA; HFSP; CTLA3
• Applications: Immunohistochemistry, Western Blot
• Synonyms: GRANZYME A; Monoclonal Antibody; MOUSE ANTI HUMAN GRANZYME A; anti-GZMA antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: GA6
• Specificity: GRANZYME A
• Form/Format: Purified IgG - liquid; Phosphate buffered saline.
• Concentration: IgG concentration 1.0 mg/ml (varies by lot)
• Sequence Length: 262

• Applicable Applications for anti-GZMA antibody: Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: Immunohistology - Paraffin: Minimum Dilution: 1/50; Maximum Dilution: 1/200; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3)
• Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
• Immunogen: Recombinant human Granzyme A; Fusion Partners
• Target Species: Human
• Fusion Partners: Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
• Note: Mouse anti Human granzyme A antibody, clone GA6 recognizes Granzyme A, a ~60 kDadisulphide-linked homodimeric protein of two 262 amino acid chains, expressed in cytoplasmicgranules of cytotoxic lymphocytes and NK cells.; ; Granzyme A is involved in the induction of apoptosis via its activity as a serine protease, but this would seem to be subsidiary to the role of Granzyme B. Granzyme A deficient mice areindistinguishable from normal animals in their response to infection. ; ; Granzyme A has been proposed as a potential biomarker for patients with active tuberculosis with significantly lower levels present in the plasma of patients with the active form of the diseasecompared to patients with latent infection (Guggino et al. 2015)
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-GZMA antibody

MBS212504 recognises Granzyme A, a 60kD disulphide-linked homodimeric protein expressed in cytoplasmic granules of cytotoxic lymphocytes. The biological function of Granzyme A is unclear. It would appear that the molecule may have a role in the induction of apoptosis via its activity as a serine protease, but this would seem to be subsidiary to the role of Granzyme B. Granzyme A deficient mice are indistinguishable from normal animals in their response to infection.

NCBI and Uniprot Product Information

• NCBI GI #: 5453676
• NCBI GeneID: 3001
• NCBI Accession #: NP_006135.1
• NCBI GenBank Nucleotide #: NM_006144.3
• UniProt Accession #: P12544; Q6IB36; A4PHN1
• NCBI Official Full Name: granzyme A
• NCBI Official Synonym Full Names: granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3)
• NCBI Official Symbol: GZMA
• NCBI Official Synonym Symbols: HFSP; CTLA3
• NCBI Protein Information: granzyme A; CTL tryptase; Granzyme A (Cytotoxic T-lymphocyte-associated serine esterase-3; Hanukah factor serine protease); HF; cytotoxic T-lymphocyte proteinase 1; fragmentin-1
• UniProt Protein Name: Granzyme A
• Protein Family: Granzyme
• UniProt Gene Name: GZMA
• UniProt Synonym Gene Names: CTLA3; HFSP; H factor; HF
• UniProt Entry Name: GRAA_HUMAN

NCBI Description

Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here is a T cell- and natural killer cell-specific serine protease that may function as a common component necessary for lysis of target cells by cytotoxic T lymphocytes and natural killer cells. [provided by RefSeq, Jul 2008]

Uniprot Description

GZMA: This enzyme is necessary for target cell lysis in cell- mediated immune responses. It cleaves after Lys or Arg. Cleaves APEX1 after 'Lys-31' and destroys its oxidative repair activity. Involved in apoptosis. Homodimer; disulfide-linked. Interacts with APEX1. Dexamethasone (DEX) induces expression of isoform beta and represses expression of isoform alpha. The alteration in expression is mediated by binding of glucocorticoid receptor to independent promoters adjacent to the alternative first exons of isoform alpha and isoform beta. Belongs to the peptidase S1 family. Granzyme subfamily. 2 isoforms of the human protein are produced by alternative promoter.

Protein type: Apoptosis; Secreted; EC 3.4.21.78; Protease; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q11-q12

Cellular Component: extracellular region; immunological synapse; nucleus

Molecular Function: protein binding; protein homodimerization activity; serine-type endopeptidase activity

Biological Process: proteolysis involved in cellular protein catabolic process; positive regulation of apoptosis; apoptosis; cytolysis; negative regulation of oxidoreductase activity; negative regulation of endodeoxyribonuclease activity; immune response; negative regulation of DNA binding