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ELISA (EIA) (Figure 1. ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human GPRC5D protein, hFc-His tagged protein can bind Rabbit anti-GPRC5D monoclonal antibody (clone: DM62) in a linear range of 1-100 ng/ml.)

Rabbit anti-Human GPRC5D Monoclonal Antibody | anti-GPRC5D antibody

Anti-GPRC5D antibody (DM62), Rabbit mAb

Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay
Purity
Purified from cell culture supernatant by affinity chromatography
Synonyms
GPRC5D; Monoclonal Antibody; Anti-GPRC5D antibody (DM62); Rabbit mAb; anti-GPRC5D antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
DM62
Purity/Purification
Purified from cell culture supernatant by affinity chromatography
Form/Format
Liquid. 50% Glycerol; PBS, pH7.4; 0.1% BSA; 0.1% Procline 300
Applicable Applications for anti-GPRC5D antibody
ELISA (EIA), Flow Cytometry (FC/FACS)
Application Notes
ELISA: 1:5000-10,000
FC/FACS: 1:100
Immunogen
Recombinant human GPRC5D (Met1-Glu27) produced by using human HEK293 cells
Reconstitution
Reconstitute with deionized water
Preparation and Storage
Store at -20 degree C for 12 months (Avoid repeated freezing and thawing)

ELISA (EIA)

(Figure 1. ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human GPRC5D protein, hFc-His tagged protein can bind Rabbit anti-GPRC5D monoclonal antibody (clone: DM62) in a linear range of 1-100 ng/ml.)

ELISA (EIA) (Figure 1. ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human GPRC5D protein, hFc-His tagged protein can bind Rabbit anti-GPRC5D monoclonal antibody (clone: DM62) in a linear range of 1-100 ng/ml.)

Flow Cytometry (FC/FACS)

(Figure 2. Flow cytometry data of serially titrated Rabbit anti- GPRC5D monoclonal antibody (clone: DM62) on H929 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)

Flow Cytometry (FC/FACS) (Figure 2. Flow cytometry data of serially titrated Rabbit anti- GPRC5D monoclonal antibody (clone: DM62) on H929 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)

Testing Data

(Figure 3. Affinity ranking of different Rabbit anti- GPRC5D mAb clones by titration of different concentration onto H929 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)

Testing Data (Figure 3. Affinity ranking of different Rabbit anti- GPRC5D mAb clones by titration of different concentration onto H929 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)
Related Product Information for anti-GPRC5D antibody
The protein encoded by this gene is a member of the G protein-coupled receptor family; however, the specific function of this gene has not yet been determined.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
38,791 Da
NCBI Official Full Name
G-protein coupled receptor family C group 5 member D
NCBI Official Synonym Full Names
G protein-coupled receptor, family C, group 5, member D
NCBI Official Symbol
GPRC5D
NCBI Protein Information
G-protein coupled receptor family C group 5 member D; orphan G-protein coupled receptor
UniProt Protein Name
G-protein coupled receptor family C group 5 member D
UniProt Gene Name
GPRC5D
UniProt Entry Name
GPC5D_HUMAN

NCBI Description

The protein encoded by this gene is a member of the G protein-coupled receptor family; however, the specific function of this gene has not yet been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

GPRC5D: is a member of the G protein-coupled receptor family; however, the specific function of this gene has not yet been determined. [provided by RefSeq, Jul 2008]

Protein type: GPCR, family 3; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 12p13.3

Cellular Component: integral to membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway

Research Articles on GPRC5D

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Product Notes

The GPRC5D gprc5d (Catalog #AAA188197) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-GPRC5D antibody (DM62), Rabbit mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GPRC5D can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS). ELISA: 1:5000-10,000 FC/FACS: 1:100. Researchers should empirically determine the suitability of the GPRC5D gprc5d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GPRC5D, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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