GPIIb/IIIa recombinant antibody
Anti-GPIIb/IIIa [10E5]
Gene Names
ITGA2B; GT; GTA; CD41; GP2B; HPA3; CD41B; GPIIb; BDPLT2; BDPLT16; PPP1R93
Reactivity
Human
Applications
ELISA, Western Blot, Immunofluorescence
Purity
Protein A Affinity Purified
Synonyms
GPIIb/IIIa; Monoclonal Recombinant Antibody; Anti-GPIIb/IIIa [10E5]; Glycoprotein IIb/IIIa; alphaIIbbeta3; Integrin alpha-IIb/beta-3; GPIIb/IIIa recombinant antibody
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG2a, Kappa
Clone Number
10E5
Specificity
The antibody binds to GPIIb/IIIa with an affinity (Kd) of 16.2+/-6 nM. The antibody completely inhibits platelet aggregation at 7.8 ug/ml.
Purity/Purification
Protein A Affinity Purified
Concentration
1 mg/ml (varies by lot)
Sequence Length
1039
Applicable Applications for GPIIb/IIIa recombinant antibody
ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes
The antibody binds specifically to human GPIIb/IIIa, and integrin complex of membrane glycoproteins expressed on the surface of platelets. The GPIIb/IIIa complex acts as a receptor for fibrinogen and Von Willebran factor, hemostatic glycoproteins that mediate platelet aggregation and blood coagulation. The monoclonal antibody 10E5 inhibits the binding of fibrinogen to its platelet receptor, preventing the platelet aggregation, to be used during coronary artery surgeries such as angioplasty. The antibody is also useful in the study of the disease Glanzmann's thrombasthenia, caused by defects in GPIIb/IIIa, and of immune thrombocytopenic purpura, where autoantibodies are produced against GPIIb/IIIa.
Immunogen
Human blood platelets.
Buffer Composition
PBS only with 0.02% Proclin 300.
Labeled Substance
None
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Related Product Information for GPIIb/IIIa recombinant antibody
Recombinant monoclonal antibody to GPIIb/IIIa. Manufactured using a proprietary Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 10E5.
Human blood platelets.
Human blood platelets.
Product Categories/Family for GPIIb/IIIa recombinant antibody
References
Coller BS, Peerschke EI, Scudder LE, Sullivan CA. A murine monoclonal antibody that completely blocks the binding of fibrinogen to platelets produces a thrombasthenic-like state in normal platelets and binds to glycoproteins IIb and/or IIIa. J Clin Invest. 1983 Jul; 72(1):325-38. PMID:6308050
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
103,218 Da
NCBI Official Full Name
integrin alpha-IIb preproprotein
NCBI Official Synonym Full Names
integrin subunit alpha 2b
NCBI Official Symbol
ITGA2B
NCBI Official Synonym Symbols
GT; GTA; CD41; GP2B; HPA3; CD41B; GPIIb; BDPLT2; BDPLT16; PPP1R93
NCBI Protein Information
integrin alpha-IIb
UniProt Protein Name
Integrin alpha-IIb
UniProt Gene Name
ITGA2B
UniProt Synonym Gene Names
GP2B; ITGAB; GPIIb
NCBI Description
This gene encodes a member of the integrin alpha chain family of proteins. The encoded preproprotein is proteolytically processed to generate light and heavy chains that associate through disulfide linkages to form a subunit of the alpha-IIb/beta-3 integrin cell adhesion receptor. This receptor plays a crucial role in the blood coagulation system, by mediating platelet aggregation. Mutations in this gene are associated with platelet-type bleeding disorders, which are characterized by a failure of platelet aggregation, including Glanzmann thrombasthenia. [provided by RefSeq, Jan 2016]
Uniprot Description
Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface.
Research Articles on GPIIb/IIIa
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Product Notes
The GPIIb/IIIa itga2b (Catalog #AAA488325) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-GPIIb/IIIa [10E5] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GPIIb/IIIa can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunofluorescence (IF). The antibody binds specifically to human GPIIb/IIIa, and integrin complex of membrane glycoproteins expressed on the surface of platelets. The GPIIb/IIIa complex acts as a receptor for fibrinogen and Von Willebran factor, hemostatic glycoproteins that mediate platelet aggregation and blood coagulation. The monoclonal antibody 10E5 inhibits the binding of fibrinogen to its platelet receptor, preventing the platelet aggregation, to be used during coronary artery surgeries such as angioplasty. The antibody is also useful in the study of the disease Glanzmann's thrombasthenia, caused by defects in GPIIb/IIIa, and of immune thrombocytopenic purpura, where autoantibodies are produced against GPIIb/IIIa. Researchers should empirically determine the suitability of the GPIIb/IIIa itga2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GPIIb/IIIa, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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