Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Rat IL6ST(CD130) expression on rat splenocytes. SD splenocytes were stained with PE-conjugated anti-Rat IL6ST(CD130). The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Rabbit anti-Rat gp130/IL6ST Monoclonal Antibody | anti-IL6ST antibody
Recombinant Anti-gp130/IL6ST Antibody (PE), Rabbit Monoclonal
Gene Names
Il6st; Gp130; Ac1055; Il-6rb
Reactivity
Rat
Applications
Flow Cytometry, Functional Assay
Purity
Protein A
Synonyms
gp130/IL6ST; Monoclonal Antibody; Recombinant Anti-gp130/IL6ST Antibody (PE); Rabbit Monoclonal; gp130/CD130/IL6ST Antibody (PE); Rabbit MAb; interleukin 6 signal transducer; anti-IL6ST antibody
Host
Rabbit
Reactivity
Rat
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
108
Specificity
Rat gp130/CD130/IL6ST
Purity/Purification
Protein A
Form/Format
Liquid; Aqueous solution containing 0.5% BSA and 0.09% sodium azide
Concentration
10ul/Test, 0.1mg/ml (varies by lot)
Applicable Applications for anti-IL6ST antibody
Flow Cytometry (FC/FACS/FCM)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Rat gp130/CD130/IL6ST protein
Conjugation
PE
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Rat gp130/CD130/IL6ST (rR gp130/CD130/IL6ST) and conjugated with PE under optimum conditions, the unreacted PE was removed.
Preparation and Storage
This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Rat IL6ST(CD130) expression on rat splenocytes. SD splenocytes were stained with PE-conjugated anti-Rat IL6ST(CD130). The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Rat IL6ST(CD130) expression on rat splenocytes. SD splenocytes were stained with PE-conjugated anti-Rat IL6ST(CD130). The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Related Product Information for anti-IL6ST antibody
Glycoprotein 130 (also known as gp130, IL6ST, IL6-beta, or CD130) is a transmembrane protein that is the founding member of the class of all cytokine receptors. CD130/gp130 is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and Oncostatin M (OSM). CD130/gp130 functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. CD130/gp130 plays a critical role in regulating myocyte apoptosis. Alternatively, spliced transcript variants encoding distinct isoforms have been described. A related pseudogene has been identified on chromosome 17. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1, and BSF3 can utilize gp130 for initiating signal transmission. CD130/gp130 binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. CD130/gp130 may have a role in embryonic development. The type I OSM receptor is capable of transducing OSM-specific signaling events.
Product Categories/Family for anti-IL6ST antibody
References
Hibi, et al. (1990) Molecular cloning and expression of an IL-6 signal transducer, gp130. Cell. 63 (6): 1149-57.Kim H, et al. (1997) Transmembrane domain of gp130 contributes to intracellular signal transduction in hepatic cells. J Biol Chem. 272 (49): 30741-7.Giordano V, et al. (1997) Shc mediates IL-6 signaling by interacting with gp130 and Jak2 kinase. J Immunol. 158 (9): 4097-103.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
102,450 Da
NCBI Official Full Name
interleukin-6 receptor subunit beta
NCBI Official Synonym Full Names
interleukin 6 signal transducer
NCBI Official Symbol
Il6st
NCBI Official Synonym Symbols
Gp130; Ac1055; Il-6rb
NCBI Protein Information
interleukin-6 receptor subunit beta
UniProt Protein Name
Interleukin-6 receptor subunit beta
Protein Family
UniProt Gene Name
Il6st
UniProt Synonym Gene Names
IL-6 receptor subunit beta; IL-6R subunit beta; IL-6R-beta; IL-6RB; gp130
NCBI Description
IL-6 signal transducing molecule [RGD, Feb 2006]
Uniprot Description
Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize IL6ST for initiating signal transmission. Binding of IL6 to IL6R induces IL6ST homodimerization and formation of a high-affinity receptor complex, which activates Janus kinases. That causes phosphorylation of IL6ST tyrosine residues which in turn activates STAT3. Mediates signals which regulate immune response, hematopoiesis, pain control and bone metabolism. Has a role in embryonic development. Does not bind IL6. Essential for survival of motor and sensory neurons and for differentiation of astrocytes. Required for expression of TRPA1 in nociceptive neurons. Required for the maintenance of PTH1R expression in the osteoblast lineage and for the stimulation of PTH-induced osteoblast differentiation. Required for normal trabecular bone mass and cortical bone composition.
Research Articles on IL6ST
Similar Products
Product Notes
The IL6ST il6st (Catalog #AAA8112662) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-gp130/IL6ST Antibody (PE), Rabbit Monoclonal reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's gp130/IL6ST can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS/FCM). Researchers should empirically determine the suitability of the IL6ST il6st for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "gp130/IL6ST, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
