Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Peroxisomal multifunctional enzyme type 2 (HSD17B4) Standard Curve.)
Human Peroxisomal Multifunctional Enzyme Type 2 (HSD17B4) ELISA Kit | HSD17B4 elisa kit
Human Peroxisomal Multifunctional Enzyme Type 2 (HSD17B4) ELISA Kit
Gene Names
HSD17B4; DBP; MFE-2; MPF-2; PRLTS1; SDR8C1
Reactivity
Human
Synonyms
Peroxisomal Multifunctional Enzyme Type 2 (HSD17B4); Human Peroxisomal Multifunctional Enzyme Type 2 (HSD17B4) ELISA Kit; HSD17B4; DBP; MFE-2; SDR8C1; 17-beta-HSD IV; 17-beta-hydroxysteroid dehydrogenase 4; 17beta-estradiol dehydrogenase type IV; 3-alpha; 7-alpha; 12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase; D-3-hydroxy; HSD17B4 elisa kit
Reactivity
Human
Specificity
Human Peroxisomal multifunctional enzyme type 2 (HSD17B4) ELISA Kit has high sensitivity and excellent specificity for detection of Human HSD17B4. No significant cross-reactivity or interference between Human HSD17B4 and analogues was observed.
Sequence Length
736
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Peroxisomal multifunctional enzyme type 2 (HSD17B4) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Peroxisomal multifunctional enzyme type 2 (HSD17B4) Standard Curve.)
Related Product Information for HSD17B4 elisa kit
Description: This Human Peroxisomal multifunctional enzyme type 2 (HSD17B4) ELISA Kit employs a two-site sandwich ELISA to quantitate HSD17B4 in samples. An antibody specific for HSD17B4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HSD17B4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HSD17B4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HSD17B4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The HSD17B4 gene encodes an enzyme involved in peroxisomal fatty acid beta-oxidation. It was first identified as a 17-beta-estradiol dehydrogenase. Peroxisomal beta-oxidation of fatty acids is catalyzed by 3 enzymes: acyl-CoA oxidase; the 'D-bifunctional enzyme,' with enoyl-CoA-hydratase and D-3-hydroxyacyl-CoA dehydrogenase activity, and 3-ketoacyl-CoA thiolase. See also the L-bifunctional peroxisomal protein. The D- and L-bifunctional proteins have different substrate specificities. The D-bifunctional protein catalyzes the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids and also acts in shortening cholesterol for bile acid formation. In contrast, the L-specific bifunctional protein does not have the latter 2 activities.
Background: The HSD17B4 gene encodes an enzyme involved in peroxisomal fatty acid beta-oxidation. It was first identified as a 17-beta-estradiol dehydrogenase. Peroxisomal beta-oxidation of fatty acids is catalyzed by 3 enzymes: acyl-CoA oxidase; the 'D-bifunctional enzyme,' with enoyl-CoA-hydratase and D-3-hydroxyacyl-CoA dehydrogenase activity, and 3-ketoacyl-CoA thiolase. See also the L-bifunctional peroxisomal protein. The D- and L-bifunctional proteins have different substrate specificities. The D-bifunctional protein catalyzes the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids and also acts in shortening cholesterol for bile acid formation. In contrast, the L-specific bifunctional protein does not have the latter 2 activities.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
peroxisomal multifunctional enzyme type 2 isoform 2
NCBI Official Synonym Full Names
hydroxysteroid 17-beta dehydrogenase 4
NCBI Official Symbol
HSD17B4
NCBI Official Synonym Symbols
DBP; MFE-2; MPF-2; PRLTS1; SDR8C1
NCBI Protein Information
peroxisomal multifunctional enzyme type 2
UniProt Protein Name
Peroxisomal multifunctional enzyme type 2
UniProt Gene Name
HSD17B4
UniProt Synonym Gene Names
EDH17B4; MFE-2; 17-beta-HSD 4; DBP; MPF-2
UniProt Entry Name
DHB4_HUMAN
NCBI Description
The protein encoded by this gene is a bifunctional enzyme that is involved in the peroxisomal beta-oxidation pathway for fatty acids. It also acts as a catalyst for the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids. Defects in this gene that affect the peroxisomal fatty acid beta-oxidation activity are a cause of D-bifunctional protein deficiency (DBPD). An apparent pseudogene of this gene is present on chromosome 8. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014]
Research Articles on HSD17B4
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Product Notes
The Human HSD17B4 hsd17b4 (Catalog #AAA9716047) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716047 ELISA Kit recognizes Human HSD17B4. It is sometimes possible for the material contained within the vial of "Peroxisomal Multifunctional Enzyme Type 2 (HSD17B4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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