SDS-Page
(The Recombinant human GOT2 (50ng), Cell and Tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with human GOT2 (1:1000). Proteins were visualized using a goat mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: GOT2 Recombinant protein Lane 2.: Ramos cell lysate Lane 3.: 293T cell lysate Lane 4.: Liver tissue lysate)
Mouse anti-Human GOT2 Monoclonal Antibody | anti-GOT2 antibody
GOT2 (Aspartate Aminotransferase 2 Mitochondrial, Aspartate Aminotransferase 2, Mitochondrial, KAT4, KATIV, mitAAT) (Glycerol/Azide free)
SDS-Page
(The Recombinant human GOT2 (50ng), Cell and Tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with human GOT2 (1:1000). Proteins were visualized using a goat mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: GOT2 Recombinant protein Lane 2.: Ramos cell lysate Lane 3.: 293T cell lysate Lane 4.: Liver tissue lysate)
SDS-Page
(The Recombinant human GOT2 (50ng), Cell and Tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with human GOT2 (1:1000). Proteins were visualized using a goat mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: GOT2 Recombinant protein Lane 2.: Ramos cell lysate Lane 3.: 293T cell lysate Lane 4.: Liver tissue lysate)
SDS-Page
(The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with human GOT2 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: HepG2 cell lysate Lane 3.: Balb/3T3 cell lysate)
SDS-Page
(The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with human GOT2 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: HepG2 cell lysate Lane 3.: Balb/3T3 cell lysate)
Flow Cytometry (FC/FACS)
(Flow cytometry analysis of GOT2 in HepG2 cell line, staining at 2-5ug for 1x10e6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).)
Flow Cytometry (FC/FACS)
(Flow cytometry analysis of GOT2 in HepG2 cell line, staining at 2-5ug for 1x10e6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).)
Immunocytochemistry (ICC)
(ICC/IF analysis of GOT2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal human GOT2 (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Immunocytochemistry (ICC)
(ICC/IF analysis of GOT2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal human GOT2 (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
NCBI and Uniprot Product Information
NCBI Description
Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2013]
Research Articles on GOT2
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Product Notes
The GOT2 got2 (Catalog #AAA6269247) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GOT2 (Aspartate Aminotransferase 2 Mitochondrial, Aspartate Aminotransferase 2, Mitochondrial, KAT4, KATIV, mitAAT) (Glycerol/Azide free) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GOT2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB). Researchers should empirically determine the suitability of the GOT2 got2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GOT2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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