Mouse GNMT Monoclonal Antibody | anti-GNMT antibody

GNMT antibody

Cat. Num. AAA533955

• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: GNMT antibody was purified by affinity chromatography.
• Synonyms: GNMT; Monoclonal Antibody; GNMT antibody; Monoclonal GNMT; Anti-GNMT; Glycine N methyltransferase; anti-GNMT antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG3
• Clone Number: 8A3
• Purity/Purification: GNMT antibody was purified by affinity chromatography.
• Form/Format: Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 500 ug-1 mg/ml (varies by lot)
• Sequence Length: 295

• Applicable Applications for anti-GNMT antibody: Flow Cytometry (FC/FACS), Western Blot (WB)
• Application Notes: FC: 1:100; WB: 1:500
• Biological Significance: GNMT is an enzyme that catalyzes the conversion of S-adenosyl-L-methionine (along withglycine) to S-adenosyl-L-homocysteine and sarcosine. The encoded protein is found in the cytoplasm and acts as ahomotetramer.
• Cross-Reactivity: Human
• Immunogen: GNMT antibody was raised in mouse using a full length recombinant protein of human GNMT (NP_061833) produced in HEK293T cells, as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant GNMT protein (Right) or empty vector (Left) detected with GNMT antibody)

Related Product Information for anti-GNMT antibody

Mouse monoclonal GNMT antibody

Product Categories/Family for anti-GNMT antibody

Proteases; Inhibitors; & Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 9506741
• NCBI GeneID: 27232
• NCBI Accession #: NP_061833
• NCBI GenBank Nucleotide #: NM_018960.4
• UniProt Accession #: Q14749; Q5T8W2; Q9NNZ1; Q9NS24
• Molecular Weight: 32,742 Da
• NCBI Official Full Name: glycine N-methyltransferase
• NCBI Official Synonym Full Names: glycine N-methyltransferase
• NCBI Official Symbol: GNMT
• NCBI Protein Information: glycine N-methyltransferase
• UniProt Protein Name: Glycine N-methyltransferase
• Protein Family: Glycine N-methyltransferase
• UniProt Gene Name: GNMT
• UniProt Entry Name: GNMT_HUMAN

NCBI Description

The protein encoded by this gene is an enzyme that catalyzes the conversion of S-adenosyl-L-methionine (along with glycine) to S-adenosyl-L-homocysteine and sarcosine. The encoded protein is found in the cytoplasm and acts as a homotetramer. Defects in this gene are a cause of GNMT deficiency (hypermethioninemia). [provided by RefSeq, Oct 2008]

Uniprot Description

GNMT: Catalyzes the methylation of glycine by using S- adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy). Possible crucial role in the regulation of tissue concentration of AdoMet and of metabolism of methionine. Defects in GNMT are the cause of glycine N- methyltransferase deficiency (GNMT deficiency); also known as hypermethioninemia. The only clinical abnormalities in patients with this deficiency are mild hepatomegaly and chronic elevation of serum transaminases. Belongs to the class I-like SAM-binding methyltransferase superfamily. Glycine N-methyltransferase family.

Protein type: Methyltransferase; Amino Acid Metabolism - glycine, serine and threonine; EC 2.1.1.20

Chromosomal Location of Human Ortholog: 6p12

Cellular Component: cytoplasm

Molecular Function: protein binding; glycine N-methyltransferase activity; glycine binding; folic acid binding

Biological Process: glycogen metabolic process; methylation; S-adenosylmethionine metabolic process; protein modification process; methionine metabolic process; regulation of gluconeogenesis; one-carbon compound metabolic process; protein homotetramerization

Disease: Glycine N-methyltransferase Deficiency