ELISA (EIA)
(Sandwich ELISA analysis of human GM-CSF using Mouse anti Human GM-CSF as a capture reagent and biotinylated Mouse anti Human GM-CSF (MBS226150) as a detection reagent with purified recombinant human GM-CSF as the antigen. Detection was performe)
Mouse anti-Human GM-CSF Monoclonal Antibody | anti-GM-CSF antibody
Mouse anti Human GM-CSF:Biotin
Gene Names
CSF2; GMCSF
Reactivity
Human
Applications
ELISA
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Synonyms
GM-CSF; Monoclonal Antibody; Mouse anti Human GM-CSF:Biotin; GM-CSF antibody | E04-5B11; anti-GM-CSF antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
E04-5B11
Purity/Purification
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Form/Format
Phosphate buffered saline
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Purified IgG conjugated to Biotin - liquid
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Purified IgG conjugated to Biotin - liquid
Concentration
IgG concentration 0.1mg/ml (varies by lot)
Applicable Applications for anti-GM-CSF antibody
ELISA (EIA)
Application Notes
ELISA: 1.0ug/ml
Immunogen
E Coli. derived recombinant human GM-CSF (Ala18-Glu144)
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
Conjugation
Biotin
Preparation and Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20 degree C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8 degree C for short term use (up to 4 weeks) and store the remaining aliquots at -20 degree C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
ELISA (EIA)
(Sandwich ELISA analysis of human GM-CSF using Mouse anti Human GM-CSF as a capture reagent and biotinylated Mouse anti Human GM-CSF (MBS226150) as a detection reagent with purified recombinant human GM-CSF as the antigen. Detection was performe)
ELISA (EIA)
(Sandwich ELISA analysis of human GM-CSF using Mouse anti Human GM-CSF as a capture reagent and biotinylated Mouse anti Human GM-CSF (MBS226150) as a detection reagent with purified recombinant human GM-CSF as the antigen. Detection was performe)
Related Product Information for anti-GM-CSF antibody
Mouse anti Human GM-CSF antibody, clone E04-5B11, recognizes Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) also known as colony stimulating factor 2. GM-CSF is a cytokine and acts as a hematopoietic grown factor and immune modulator of various immune cells. It is produced by immune cells including T cells and macrophages as well as endothelial and fibroblast cells. GM-CSF has various functions such as roles in recruiting circulating neutrophils, monocytes and lymphocytes (Shi et al. 2006). It is also involved in the maturation and activation of neutrophils and macrophages to induce microbial activity and the production of cytokines. GM-CSF elicits these functions through signaling via its receptor GM-CSFR to activate NF-kappaB or STAT5 signaling pathways (Dougan et al. 2019).
The biotinylated Mouse anti Human GM-CSF antibody, clone E04-5B11 can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human GM-CSF antibody, clone E01-1B6 as the capture antibody.
The biotinylated Mouse anti Human GM-CSF antibody, clone E04-5B11 can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human GM-CSF antibody, clone E01-1B6 as the capture antibody.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
16,295 Da
NCBI Official Full Name
GM-CSF
NCBI Official Synonym Full Names
colony stimulating factor 2 (granulocyte-macrophage)
NCBI Official Symbol
CSF2
NCBI Official Synonym Symbols
GMCSF
NCBI Protein Information
granulocyte-macrophage colony-stimulating factor; CSF; molgramostin; sargramostim
UniProt Protein Name
Granulocyte-macrophage colony-stimulating factor
UniProt Gene Name
CSF2
UniProt Synonym Gene Names
GMCSF; GM-CSF; CSF
UniProt Entry Name
CSF2_HUMAN
Similar Products
Product Notes
The GM-CSF csf2 (Catalog #AAA226150) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti Human GM-CSF:Biotin reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GM-CSF can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). ELISA: 1.0ug/ml. Researchers should empirically determine the suitability of the GM-CSF csf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GM-CSF, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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