Rat anti-Human GM-CSF Monoclonal Antibody | anti-CSF2 antibody

Rat Anti-Human GM-CSF-LE/AF

Cat. Num. AAA670025

• Gene Names: CSF2; GMCSF; MGC131935; MGC138897
• Reactivity: Human
• Applications: ELISA, Immunoprecipitation, Western Blot
• Synonyms: GM-CSF; Monoclonal Antibody; Rat Anti-Human GM-CSF-LE/AF; Rat Anti-Human GM-CSF - Low Endotoxin/Azide-Free; Rat Anti-Human GM-CSF; anti-CSF2 antibody

• Host: Rat
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rat IgG2a kappa
• Clone Number: BVD2-23B6
• Specificity: Human granulocyte/macrophage - colony stimulating factor (GM-CSF)1-4; (This is a neutralizing antibody)
• Form/Format: Low Endotoxin, Azide - Free (LE/AF)
• Sequence Length: 144

• Applicable Applications for anti-CSF2 antibody: ELISA; Immunoprecipitation; Western Blot
• Application Notes: Research Application(s): ELISA Capture: Purified BVD2-23B6 monoclonal antibody is the preferred capture antibody in a sandwich ELISA for quantifying human GM-CSF protein levels.1-4 Purified BVD2-23B6 antibody should be paired with biotinylated BVD2-21C11 antibody as the detection antibody, with purified recombinant human GM-CSF as the standard.; ; Immunoprecipitation / Western Blotting: The purified BVD2-23B6 antibody is also useful for immunoprecipitation and Western blotting applications. For Western blotting, a concentration of 1-5 ug/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of <= 100 ng/lane of recombinant human GM-CSF under reducing conditions.; ; Neutralization: The LE/AF form of BVD2-23B6 can be used for neutralizing human GM-CSF bioactivity. A suitable LE/AF Rat IgG2a should be used as an isotype control.; ; Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications.
• Immunogen: E. coli-expressed human GM-CSF
• Characterization: To ensure acceptable performance, each batch of product is tested in a sandwich ELISA to conform to characteristics of a standard reference reagent.
• Preparation and Storage: The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. ; The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C. ; Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.

References

1. Bacchetta, R., T. de Wall Malefyt, H. Yssel, J.S. Abrams, J.E. de Vries, H. Spits, and M.G. Roncarolo. 1990. J. Immunol. 144:902.
2. Kita, H., T. Ohnishi, Y. Okubo, D. Weller, J.S. Abrams, and G.J. Gleich. 1991. J. Exp. Med. 174:745.
3. Abrams, J.S., M.-G. Roncarolo, H.. Yssel, U. Andersson, G.J. Gleich, and J.E., Silver. 1992. Immunol. Rev. 127:5.
4. Abrams, J., J. Silver, R. van Dyke, and G. Gleich. 1994. Eosinophil-active cytokines in human disease: Development and use of monoclonal antibodies to IL-3-, IL-5, GM-CSF. In Eosinophils in Allergy and Inflammation (A. Kay and G. Gleich, eds). Marcel Dekker, New York, pp. 133.

NCBI and Uniprot Product Information

• NCBI GI #: 183364
• NCBI GeneID: 1437
• UniProt Accession #: P04141; Q14CE8; Q2VPI8; Q8NFI6; Q647J8
• Molecular Weight: 16,295 Da
• NCBI Official Full Name: GM-CSF
• NCBI Official Synonym Full Names: colony stimulating factor 2 (granulocyte-macrophage)
• NCBI Official Symbol: CSF2
• NCBI Official Synonym Symbols: GMCSF; MGC131935; MGC138897
• NCBI Protein Information: granulocyte-macrophage colony-stimulating factor; CSF; molgramostin; sargramostim; OTTHUMP00000065962; granulocyte-macrophage colony stimulating factor
• UniProt Protein Name: Granulocyte-macrophage colony-stimulating factor
• Protein Family: Granulocyte-macrophage colony-stimulating factor
• UniProt Gene Name: CSF2
• UniProt Synonym Gene Names: GMCSF
• UniProt Entry Name: CSF2_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq]

Uniprot Description

Function: Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.

Subunit structure: Monomer.

Subcellular location: Secreted.

Polymorphism: Variant Ile-117 may be a risk factor for atopic asthma.

Pharmaceutical use: Available under the names Leukine (Immunex) and Leucomax (Novartis). Used in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia.

Sequence similarities: Belongs to the GM-CSF family.