Rat anti-Human GM-CSF Monoclonal Antibody | anti-CSF2 antibody

Rat Anti-Human GM-CSF-UNLB

Cat. Num. AAA670026

• Gene Names: CSF2; GMCSF; MGC131935; MGC138897
• Reactivity: Human
• Applications: ELISA, Flow Cytometry, Immunofluorescence
• Synonyms: GM-CSF; Monoclonal Antibody; Rat Anti-Human GM-CSF-UNLB; Rat Anti-Human GM-CSF - Purified; Unlabeled Antibody; Rat Anti-Human GM-CSF; anti-CSF2 antibody

• Host: Rat
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rat IgG2a
• Clone Number: BVD2-21C11
• Specificity: Human granulocyte/macrophage - colony stimulating factor (GM-CSF)1-4 The BVD2-21C11 antibody has been reported to cross react with GM-CSF from Rhesus monkey. (This is a neutralizing antibody.)
• Form/Format: Purified (UNLB) Antibody
• Sequence Length: 144

• Applicable Applications for anti-CSF2 antibody: ELISA; Flow Cytometry; Immunofluorescence
• Application Notes: Research Application(s): ELISA Detection: Biotinylated BVD2-21C11 monoclonal antibody is the preferred detection antibody in a sandwich ELISA for quantifying human GM-CSF protein levels.1-4 Biotinylated BVD2-21C11antibody should be paired with purified BVD2-23B6 antibody as the capture antibody, with purified recombinant human GM-CSF as the standard.; ; Immunofluorescence/Flow Cytometry: BVD2-2C11 antibodies are useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate GM-CSF-positive cells within mixed cell populations. Recombinant human GM-CSF, unlabeled BVD2-21C11, or Rat IgG2a should be used as a specificity control.; ; Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications.
• Immunogen: E. coli-expressed human GM-CSF
• Characterization: To ensure acceptable performance, each batch of product is tested in a sandwich ELISA to conform to characteristics of a standard reference reagent.
• Preparation and Storage: The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. ; The biotin (BIOT) conjugate is supplied as 0.5 mg labeled antibody in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; Reagents are stable for the period shown on the label if stored as directed.

References

1. Abrams, J.S., M.-G. Roncarolo, H.. Yssel, U. Andersson, G.J. Gleich, and J.E., Silver. 1992. Strategies of anti-cytokine monoclonal antibody development: Immunoassay of IL-10 and IL-5 in clinical samples. Immunol. Rev. 127:5-24.
2. Abrams, J., J. Silver, R. van Dyke, and G. Gleich. 1994. Eosinophil-active cytokines in human disease: Development and use of monoclonal antibodies to IL-3-, IL-5, GM-CSF. In Eosinophils in Allergy and Inflammation (A. Kay and G. Gleich, eds). Marcel Dekker, New York, pp. 133-157.
3. Bacchetta, R., T. de Wall Malefyt, H. Yssel, J.S. Abrams, J.E. de Vries, H. Spits, and M.G. Roncarolo. 1990, Host reactive CD4+ and CD8+ T cells clones isolated from a human chimera produce IL-5, Il-2, IFN-gamma and granulocyte/macrophage stimulating factor bit not IL-4. J. Immunol. 144:902-908.
4. Kita, H., T. Ohnishi, Y. Okubo, D. Weller, J.S. Abrams, and G.J. Gleich. 1991. GM-CSF and interleukin-3 release by human peripheral blood eosinophils and neutrophils. J. Exp. Med. 174:745-748.

NCBI and Uniprot Product Information

• NCBI GI #: 183364
• NCBI GeneID: 1437
• UniProt Accession #: P04141; Q14CE8; Q2VPI8; Q8NFI6; Q647J8
• Molecular Weight: 16,295 Da
• NCBI Official Full Name: GM-CSF
• NCBI Official Synonym Full Names: colony stimulating factor 2 (granulocyte-macrophage)
• NCBI Official Symbol: CSF2
• NCBI Official Synonym Symbols: GMCSF; MGC131935; MGC138897
• NCBI Protein Information: granulocyte-macrophage colony-stimulating factor; CSF; molgramostin; sargramostim; OTTHUMP00000065962; granulocyte-macrophage colony stimulating factor
• UniProt Protein Name: Granulocyte-macrophage colony-stimulating factor
• Protein Family: Granulocyte-macrophage colony-stimulating factor
• UniProt Gene Name: CSF2
• UniProt Synonym Gene Names: GMCSF
• UniProt Entry Name: CSF2_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq]

Uniprot Description

Function: Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.

Subunit structure: Monomer.

Subcellular location: Secreted.

Polymorphism: Variant Ile-117 may be a risk factor for atopic asthma.

Pharmaceutical use: Available under the names Leukine (Immunex) and Leucomax (Novartis). Used in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia.

Sequence similarities: Belongs to the GM-CSF family.