Mouse Glutathione S-Transferase Mu2 (GSTM2) Monoclonal Antibody | anti-GSTM2 antibody

Glutathione S-Transferase Mu2 (GSTM2)

Cat. Num. AAA4380580

• Gene Names: GSTM2; GST4; GSTM; GTHMUS; GSTM2-2
• Reactivity: Human. Others not tested.
• Applications: Immunofluorescence, Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Glutathione S-Transferase Mu2 (GSTM2); Monoclonal Antibody; Glutathione S alkyltransferase M2; Glutathione S transferase 4; GST class mu 2; GST4; GST muscle; anti-GSTM2 antibody

• Host: Mouse
• Reactivity: Human. Others not tested.
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: CPTC-GSTMu2-2
• Specificity: Members of the glutathione S-transferase (GST) family of proteins function in the detoxification of xenobiotics to protect cells against toxicant-induced damage. There are eight families of GST proteins, namely alpha, zeta, theta, kappa, u, pi, sigma and omega, each of which are composed of proteins that have a variety of functions throughout the cell. The GSTM proteins (GSTM1-GSTM5 in human and GSTM1-GSTM7 in mouse) are members of the mu class of enzymes that conjugate with glutathione and function in the detoxification of carcinogens, environmental toxins and products of oxidative stress. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. Null mutations of this class mu gene have been linked with an increase in a number of cancers, likely due to an increased susceptibility to environmental toxins and carcinogens. Multiple protein isoforms are encoded by transcript variants of this gene. GSTM2 is specific for muscle hence the alternate name GST muscle antibody.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 191

• Applicable Applications for anti-GSTM2 antibody: Immunofluorescence (IF), Immunohistology (Formalin-Fixed)
• Application Notes: IF: 0.5-1ug/ml; Immunohistology (Formalin-Fixed): 0.5-1ug/ml for 30 min at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
• Immunogen: Recombinant human full-length GSTMu2 protein
• Positive Control: HeLA cell lysate; skeletal muscle extract; Kidney
• Cellular Localization: Nuclear
• Chromosome Location: 1p13.3
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C.; Antibody without azide - store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

References

Raza, H., et al. 2002. Biochem. J. 366: 45-55.

NCBI and Uniprot Product Information

• NCBI GI #: 4504175
• NCBI GeneID: 2946
• NCBI Accession #: NP_000839.1
• NCBI GenBank Nucleotide #: NM_000848.3
• UniProt Accession #: P28161; Q2M318; Q5TZY5; Q8WWE1; B4DRY4; E9PEM9
• Molecular Weight: Predicted: 26kDa; Observed: 28kDA
• NCBI Official Full Name: glutathione S-transferase Mu 2 isoform 1
• NCBI Official Synonym Full Names: glutathione S-transferase mu 2
• NCBI Official Symbol: GSTM2
• NCBI Official Synonym Symbols: GST4; GSTM; GTHMUS; GSTM2-2
• NCBI Protein Information: glutathione S-transferase Mu 2
• UniProt Protein Name: Glutathione S-transferase Mu 2
• Protein Family: Glutathione S-transferase
• UniProt Gene Name: GSTM2
• UniProt Synonym Gene Names: GST4

NCBI Description

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. [provided by RefSeq, Jul 2008]

Uniprot Description

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.