Rat anti-Mouse GLUT2 Monoclonal Antibody | anti-Slc2a2 antibody

GLUT2 (Solute Carrier Family 2, Facilitated Glucose Transporter Member 2, Glucose Transporter Type 2, Liver, GLUT-2, SLC2A2)

Cat. Num. AAA609124

• Gene Names: Slc2a2; Glut2; Glut-2; AI266973
• Reactivity: Mouse
• Applications: Flow Cytometry, Western Blot
• Purity: Affinity Purified; Purified by Protein A/G affinity chromatography
• Synonyms: GLUT2; Monoclonal Antibody; GLUT2 (Solute Carrier Family 2; Facilitated Glucose Transporter Member 2; Glucose Transporter Type 2; Liver; GLUT-2; SLC2A2); Anti -GLUT2 (Solute Carrier Family 2; anti-Slc2a2 antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 11C102
• Specificity: Recognizes mouse GLUT2.
• Purity/Purification: Affinity Purified; Purified by Protein A/G affinity chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.5
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-Slc2a2 antibody: Suitable for use in Western Blot and Flow Cytometry. Other application not tested.
• Application Notes: Dilution: Western Blot: 1-5ug/ml (ECL). Do not to heat the samples prior to loading to prevent gluts aggregation. Mouse tissues or mouse insulinoma beta TC-6 cells can be used as positive control.; ; Flow Cytometry: ~10ug/ml (10e6 cells) Perform the antibody binding in small volume of ~200ul.; ; Optimals dilutions to be determined by the researcher.
• Immunogen: Recombinant corresponding to mouse GLUT2. Epitope: Extracellular domain
• Preparation and Storage: May be stored at 4°C for short term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-Slc2a2 antibody

Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (Glut-1- Glut-14, Mol. Wt. 40-80kD) and Sodium glucose co-transporter- 1 (SGLT-1, 662aa; ~75kD). Individual member of this family have identical predicted secondary structures with 12 transmembrane domains. Both N and c-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting. Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics. Glut-1 mediates glucose transport into red cells, and throughout the blood brain barrier, and supply glucose to most cells. Glut-2 provides glucose to the liver and pancreatic cells. Glut-3 is the main transporter in neurons, whereas Glut-4 is primarily expressed in muscle and adipose tissue and regulated by insulin. Glut-5 transports fructose in intestine and testis.

Glut-6 a pseudoegene and unlikely to be expressed at the protein level. Glut-7 expressed in liver and other gluconeogenic tissues, mediates glucose flux across endoplasmic reticulum membrane. Glut-8 is found in adult testis and placenta. Human Glut-9 is expressed in spleen, peripheral leukocytes and brain. Human Glut-10 (541aa, chromosome 20q13.1;~30-35% homology with Glut-3 and Glut 8) has been identified as a candidate gene for NIDDM susceptibility. It is widely expressed in heart and skeletal muscle Glut-12 (human-617aa,,monkey 621aa;~~50kD; ~30% homology with Glut-4 and 40% with Glut-10) is expressed in skeletal muscle, adipose tissue, and small intestine.

Product Categories/Family for anti-Slc2a2 antibody

Antibodies; Abs to Glucose Transporters

NCBI and Uniprot Product Information

• NCBI GI #: 683589
• NCBI GeneID: 20526
• UniProt Accession #: P14246; Q3TNA5; Q9DBA7
• NCBI Official Full Name: GLUT2
• NCBI Official Synonym Full Names: solute carrier family 2 (facilitated glucose transporter), member 2
• NCBI Official Symbol: Slc2a2
• NCBI Official Synonym Symbols: Glut2; Glut-2; AI266973
• NCBI Protein Information: solute carrier family 2, facilitated glucose transporter member 2; liver-type glucose transporter; glucose transporter type 2, liver
• UniProt Protein Name: Solute carrier family 2, facilitated glucose transporter member 2
• Protein Family: Solute carrier family
• UniProt Gene Name: Slc2a2
• UniProt Synonym Gene Names: Glut-2; Glut2
• UniProt Entry Name: GTR2_MOUSE

Uniprot Description

GLUT2: an integral membrane facilitative glucose transporter. One of 13 members of the human equilibrative glucose transport protein family. Present at high levels in the plasma membrane of pancreatic b-cells where it initiates the first step in glucose-stimulated insulin secretion, a glucose metabolism-dependent signaling mechanism. May mediate the bidirectional transfer of glucose across the plasma membrane of hepatocytes. May also participate with the Na( +2)/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney. Required for glucose sensitivity in the hypothalamus and brain stem. Involved in the control of food intake and stimulation of glucose uptake by peripheral tissues.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transporter, SLC family; Transporter

Cellular Component: membrane; basolateral plasma membrane; brush border membrane; cytoplasm; plasma membrane; integral to membrane; cytosol; brush border; endosome

Molecular Function: D-glucose transmembrane transporter activity; dehydroascorbic acid transporter activity; transporter activity; glucose transmembrane transporter activity; transmembrane transporter activity; substrate-specific transmembrane transporter activity; insulin receptor binding

Biological Process: carbohydrate utilization; transport; carbohydrate transport; glucose transport; transmembrane transport