Related Product Information for anti-NR3C1 antibody
Mouse anti Human glucocorticoid receptor antibody, clone 8E9 recognizes the human glucocorticoid receptor (GR), also known as Nuclear receptor subfamily 3 group C member 1. Human GR is a 777 amino acid ~97kDa (Moraes et al. 2005) member of the NR3 subfamily of nuclear hormone receptors, bearing a single nuclear receptor DNA-binding domain. Multiple isoforms of the human glucocorticoid receptor are generated by either alternative splicing or alternative initiation (UniProt:: P04150). In the absence of bound ligand GRs are located in the cytoplasm and are translocated to the nucleus or mitochondrion following ligation (Rossini et al. 1984). GRs are associated with heat shock proteins in the cytoplasm when ligated to steroid hormone, being disrupted on translocation of the steroid:receptor complex to the nucleus (Tse et al. 2011). Mouse anti Human glucocorticoid receptor antibody, clone 8E9 was raised against a conserved region of the glucocorticoid receptor and recognizes human GR, binding to an epitope between amino acids 167-176 and is therefore expected to bind all described isoforms of the human glucocorticoid receptor. Mouse anti Human glucocorticoid receptor antibody, clone 8E9 has been used successfully for the identification of human glucocorticoid receptor using flow cytometry (Berki et al. 1998), western blotting (Moraes et al. 2005) and immunoprecipitation where it has also been shown to bind to the murine GR (Paul-Clark et al. 2003, Bartis et al. 2007).
NCBI Description
This gene encodes glucocorticoid receptor, which can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors. This receptor is typically found in the cytoplasm, but upon ligand binding, is transported into the nucleus. It is involved in inflammatory responses, cellular proliferation, and differentiation in target tissues. Mutations in this gene are associated with generalized glucocorticoid resistance. Alternative splicing of this gene results in transcript variants encoding either the same or different isoforms. Additional isoforms resulting from the use of alternate in-frame translation initiation sites have also been described, and shown to be functional, displaying diverse cytoplasm-to-nucleus trafficking patterns and distinct transcriptional activities (PMID:15866175). [provided by RefSeq, Feb 2011]
Uniprot Description
GR: transcription factor of the nuclear receptor family. Receptor for glucocorticoids (GC). Binds to glucocorticoid response elements (GRE) and modulates other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Three alternatively spliced isoforms have been described.
Protein type: Mitochondrial; Nuclear receptor; Transcription factor; DNA-binding
Chromosomal Location of Human Ortholog: 5q31.3
Cellular Component: nucleoplasm; membrane; mitochondrial matrix; cytoplasm; cytosol; nucleus
Molecular Function: protein dimerization activity; glucocorticoid receptor activity; protein binding; zinc ion binding; transcription factor activity; steroid binding
Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; maternal behavior; adrenal gland development; regulation of glucocorticoid biosynthetic process; chromatin modification; signal transduction; glucocorticoid mediated signaling; glucocorticoid metabolic process; regulation of transcription, DNA-dependent; positive regulation of neuron apoptosis; glucocorticoid receptor signaling pathway; gene expression; positive regulation of transcription from RNA polymerase II promoter; regulation of gluconeogenesis
Disease: Glucocorticoid Resistance, Generalized
Product Notes
The NR3C1 nr3c1 (Catalog #
AAA212124) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI HUMAN GLUCOCORTICOID RECEPTOR reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GLUCOCORTICOID RECEPTOR can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). ELISA: Maximum Dilution: 1/1000; Western Blotting: Maximum Dilution: 1/1000. Researchers should empirically determine the suitability of the NR3C1 nr3c1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
It is sometimes possible for the material contained within the vial of
"GLUCOCORTICOID RECEPTOR, Monoclonal Antibody" to become dispersed throughout the inside of
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