Western Blot (WB)
(Western blot analysis of lysates from HT29, HepG2 cell line (from left to right), using Glucagon Antibody (C-term). MBS9200310 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Mouse anti-Human Glucagon Monoclonal Antibody | anti-GCG antibody
Glucagon Antibody
Western Blot (WB)
(Western blot analysis of lysates from HT29, HepG2 cell line (from left to right), using Glucagon Antibody (C-term). MBS9200310 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Western Blot (WB)
(Western blot analysis of lysates from HT29, HepG2 cell line (from left to right), using Glucagon Antibody (C-term). MBS9200310 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
preproprotein that is cleaved into four distinct mature peptides.
One of these, glucagon, is a pancreatic hormone that counteracts
the glucose-lowering action of insulin by stimulating
glycogenolysis and gluconeogenesis. Glucagon is a ligand for a
specific G-protein linked receptor whose signalling pathway
controls cell proliferation. Two of the other peptides are secreted
from gut endocrine cells and promote nutrient absorption through
distinct mechanisms. Finally, the fourth peptide is similar to
glicentin, an active enteroglucagon.
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Hare, K.J. Dan Med Bull 57 (9), B4181 (2010) :
Yamaoka-Tojo, M., et al. Cardiovasc Diabetol 9, 17 (2010) :
Bertenshaw, G.P., et al. J. Biol. Chem. 276(16):13248-13255(2001)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is actually a preproprotein that is cleaved into four distinct mature peptides. One of these, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signalling pathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promote nutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an active enteroglucagon. [provided by RefSeq, Jul 2008]
Uniprot Description
GCG: Glucagon plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes. Glucagon release is stimulated by hypoglycemia and inhibited by hyperglycemia, insulin, and somatostatin. GLP-1 and GLP-2 are induced in response to nutrient ingestion. Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP1 and GLP2 are also secreted in selected neurons in the brain. Belongs to the glucagon family.
Protein type: Secreted; Hormone; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 2q36-q37
Cellular Component: extracellular space; endoplasmic reticulum lumen; plasma membrane; extracellular region
Molecular Function: identical protein binding; protein binding; hormone activity; glucagon receptor binding; receptor binding
Biological Process: positive regulation of protein binding; positive regulation of histone H3-K4 methylation; signal transduction; positive regulation of peptidyl-serine phosphorylation; G-protein signaling, coupled to cAMP nucleotide second messenger; response to starvation; cell proliferation; G-protein coupled receptor protein signaling pathway; cellular protein metabolic process; positive regulation of protein kinase activity; positive regulation of cAMP biosynthetic process; energy reserve metabolic process; feeding behavior; regulation of insulin secretion; negative regulation of apoptosis
Research Articles on GCG
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Product Notes
The GCG gcg (Catalog #AAA9200310) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 119-148. The Glucagon Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Glucagon can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the GCG gcg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glucagon, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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