Mouse anti-Human GGA1 Monoclonal Antibody | anti-GGA1 antibody

GGA1 (ADP-ribosylation Factor-binding Protein GGA1, Gamma-adaptin-related Protein 1, Golgi-localized, gamma Ear-containing, ARF-binding Protein 1) (FITC)

Cat. Num. AAA6147367

• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GGA1; Monoclonal Antibody; GGA1 (ADP-ribosylation Factor-binding Protein GGA1; Gamma-adaptin-related Protein 1; Golgi-localized; gamma Ear-containing; ARF-binding Protein 1) (FITC); anti-GGA1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 3F11
• Specificity: Recognizes human GGA1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).

• Applicable Applications for anti-GGA1 antibody: ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC-P: 3ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-90 from human GGA1 (AAH29388) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MEPAMEPETLEARINRATNPLNKELDWASINGFCEQLNEDFEGPPLATRLLAHKIQSPQEWEAIQALTVRRGEATIRPPPCDDTKGGQD*
• Conjugate: FITC
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (35.9kD).)

Western Blot (WB)

(GGA1 monoclonal antibody. Western Blot analysis of GGA1 expression in K-562.)

Immunohistochemistry (IHC)

(Immunoperoxidase of monoclonal antibody to GGA1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3ug/ml])

Testing Data

(Detection limit for recombinant GST tagged GGA1 is ~0.03ng/ml as a capture antibody.)

Product Categories/Family for anti-GGA1 antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 20810200
• NCBI GeneID: 26088
• Molecular Weight: 72,277 Da
• NCBI Official Full Name: Homo sapiens golgi associated, gamma adaptin ear containing, ARF binding protein 1, mRNA
• NCBI Official Synonym Full Names: golgi associated, gamma adaptin ear containing, ARF binding protein 1
• NCBI Official Symbol: GGA1
• NCBI Protein Information: ADP-ribosylation factor-binding protein GGA1
• Protein Family: ADP-ribosylation factor-binding protein

NCBI Description

This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) protein family. Members of this family are ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]