Western Blot (WB)
(Western BlotPositive WB detected in: 50ng recombinant proteinAll lanes: GFP antibody at 1:50000, 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000, 1:3200000, 1:6400000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 32 KDaObserved band size: 32 KDaExposure time?5min)
Mouse anti-All GFP Monoclonal Antibody | anti-GFP antibody
GFP Monoclonal Antibody
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH7.4
IF: 1:50-1:200
FC: 1:100-1:300
IP: 1ul-2ul
Western Blot (WB)
(Western BlotPositive WB detected in: 50ng recombinant proteinAll lanes: GFP antibody at 1:50000, 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000, 1:3200000, 1:6400000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 32 KDaObserved band size: 32 KDaExposure time?5min)
Western Blot (WB)
(Western BlotPositive WB detected in: 50ng recombinant proteinAll lanes: GFP antibody at 1:50000, 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000, 1:3200000, 1:6400000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 32 KDaObserved band size: 32 KDaExposure time?5min)
Western Blot (WB)
(Western BlotPositive WB detected in: Recombinant protein at 25ng, 12.5ng, 6.25ng, 3.125ng, 1.56ng, 0.78ng, 0.39ng, 0.195ngAll lanes: GFP antibody at 1:2000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 32 KDaObserved band size: 32 KDaExposure time?5min)
Western Blot (WB)
(Western BlotPositive WB detected in: Recombinant protein at 25ng, 12.5ng, 6.25ng, 3.125ng, 1.56ng, 0.78ng, 0.39ng, 0.195ngAll lanes: GFP antibody at 1:2000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 32 KDaObserved band size: 32 KDaExposure time?5min)
Western Blot (WB)
(Western BlotPositive WB detected in: 1,2,3 and 4 are Recombinant proteins with GFP tag for 50ng; 5, 293F whole cell lysate transfected with GFP for 5ugAll lanes GFP antibody at 1:5000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size:1,2,3,4 and 5 is 32,32,50,32,32 KDa respectivelyObserved band size: 32,32,50,32,32 KDaExposure time?1min)
Western Blot (WB)
(Western BlotPositive WB detected in: 1,2,3 and 4 are Recombinant proteins with GFP tag for 50ng; 5, 293F whole cell lysate transfected with GFP for 5ugAll lanes GFP antibody at 1:5000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size:1,2,3,4 and 5 is 32,32,50,32,32 KDa respectivelyObserved band size: 32,32,50,32,32 KDaExposure time?1min)
Immunofluorescence (IF)
(Immunofluorescence staining of 293F cells transfected with GFP with MBS9018370 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was R-PE-conjugated Goat Anti-Mouse IgG(H+L).)
Immunofluorescence (IF)
(Immunofluorescence staining of 293F cells transfected with GFP with MBS9018370 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was R-PE-conjugated Goat Anti-Mouse IgG(H+L).)
Flow Cytometry (FC/FACS)
(Two-color flow cytometric analysis showing 293F cells untransfected (Left) or transfected with GFP (Right) stained with MBS9018370 at 1:200. The cells were fixed in 70% ethanol at 4 degree C overnight. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 1 h at 4 degree C. The secondary antibody used was Alexa Fluor 647 AffiniPure Donkey Anti-Mouse IgG (H+L) at 1/250 dilution for 30min at 4 degree C.)
Flow Cytometry (FC/FACS)
(Two-color flow cytometric analysis showing 293F cells untransfected (Left) or transfected with GFP (Right) stained with MBS9018370 at 1:200. The cells were fixed in 70% ethanol at 4 degree C overnight. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 1 h at 4 degree C. The secondary antibody used was Alexa Fluor 647 AffiniPure Donkey Anti-Mouse IgG (H+L) at 1/250 dilution for 30min at 4 degree C.)
Flow Cytometry (FC/FACS)
(Overlay histogram showing 293F cells transfected with GFP stained with MBS9018370 (red line) at 1:200. The cells were fixed in 70% ethanol at 4 degree C overnight. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 1 h at 4 degree C. The secondary antibody used was Alexa Fluor 647 AffiniPure Donkey Anti-Mouse IgG (H+L) at 1/250 dilution for 30min at 4 degree C. Isotype control antibody (green line) was mouse IgG2b (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
Flow Cytometry (FC/FACS)
(Overlay histogram showing 293F cells transfected with GFP stained with MBS9018370 (red line) at 1:200. The cells were fixed in 70% ethanol at 4 degree C overnight. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 1 h at 4 degree C. The secondary antibody used was Alexa Fluor 647 AffiniPure Donkey Anti-Mouse IgG (H+L) at 1/250 dilution for 30min at 4 degree C. Isotype control antibody (green line) was mouse IgG2b (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
Immunoprecipitation (IP)
(Immunoprecipitating GFP in 293F whole cell lysate transfected with GFPLane 1: Mouse control IgG2b instead of MBS9018370 in 293F whole cell lysate transfected with GFPLane 2: MBS9018370 (4ug) + 293F whole cell lysate transfected with GFP (500ug)Lane 3: 293F whole cell lysate transfected with GFP (5ug)For western blotting, the blot was detected with MBS9018370 at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000)
Immunoprecipitation (IP)
(Immunoprecipitating GFP in 293F whole cell lysate transfected with GFPLane 1: Mouse control IgG2b instead of MBS9018370 in 293F whole cell lysate transfected with GFPLane 2: MBS9018370 (4ug) + 293F whole cell lysate transfected with GFP (500ug)Lane 3: 293F whole cell lysate transfected with GFP (5ug)For western blotting, the blot was detected with MBS9018370 at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000)
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Product Notes
The GFP (Catalog #AAA9018370) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GFP Monoclonal Antibody reacts with All and may cross-react with other species as described in the data sheet. AAA Biotech's GFP can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunoprecipitation (IP). WB: 1:50000-1:6400000 IF: 1:50-1:200 FC: 1:100-1:300 IP: 1ul-2ul. Researchers should empirically determine the suitability of the GFP for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GFP, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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