Mouse GFAP Monoclonal Antibody | anti-GFAP antibody

GFAP (Astrocyte or Intermediate Filament Protein, Glial Fibrillary Acidic Protein) (Biotin)

Cat. Num. AAA6124212

• Reactivity: Bovine, Chicken, Human, Mouse, Porcine, Rabbit, Rat
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: GFAP; Monoclonal Antibody; GFAP (Astrocyte or Intermediate Filament Protein; Glial Fibrillary Acidic Protein) (Biotin); Astrocyte or Intermediate Filament Protein; Glial Fibrillary Acidic Protein; anti-GFAP antibody

• Host: Mouse
• Reactivity: Bovine, Chicken, Human, Mouse, Porcine, Rabbit, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: SPM248
• Specificity: Recognizes GFAP. Species Crossreactivity: Human, Mouse, Rat, Bovine, Porcine, Rabbit, Chicken.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with Biotin.

• Applicable Applications for anti-GFAP antibody: ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: IP: 1-2ug/500ug protein lysate; FC/FACS: 0.5-1ug/million cells; IHC: 0.5-1ug/ml for 30 minutes at RT; IF: 1-2ug/ml; WB: 0.5-1ug/ml; Applications are based on unconjugated antibody.
• Immunogen: GFAP isolated from pig spinal cord. Cellular Localization: Cytoplasmic
• Conjugate: Biotin
• Positive Control: Brain or Astrocytoma
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Formalin-paraffin human brain stained with GFAP MAb (SPM248). Note cytoplasmic staining.)

Western Blot (WB)

(Western blot analysis of GFAP in human brain lysate using GFAP Ab (SPM248).)

Product Categories/Family for anti-GFAP antibody

Antibodies; Growth Factors; Cytokines

References

1. Herpers MJ et. Al. 1986, Acta Neuropathol, 70:333-339. 2. Van Muijen GN et. al. 1987, Lab Invest, 57:359-369. 3. Debus E, et. al. 1983, Differentiation, 25(2):193-203.

NCBI and Uniprot Product Information

• NCBI GI #: 196115290
• NCBI GeneID: 2670
• NCBI Accession #: NP_001124491.1
• NCBI GenBank Nucleotide #: NM_001131019.2
• UniProt Accession #: P14136; P03995; P47819
• Molecular Weight: 49,880 Da
• NCBI Official Full Name: glial fibrillary acidic protein isoform 2
• NCBI Official Synonym Full Names: glial fibrillary acidic protein
• NCBI Official Symbol: GFAP
• NCBI Protein Information: glial fibrillary acidic protein
• UniProt Protein Name: Glial fibrillary acidic protein
• Protein Family: Glial fibrillary acidic protein
• UniProt Gene Name: GFAP
• UniProt Synonym Gene Names: GFAP
• UniProt Entry Name: GFAP_HUMAN

NCBI Description

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]

Uniprot Description

GFAP: a class-III intermediate filament protein. A cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant isoform has been described, but its full length sequence has not been determined.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: membrane; cytoplasm; intermediate filament; cytosol

Molecular Function: integrin binding; structural constituent of cytoskeleton; kinase binding

Biological Process: Bergmann glial cell differentiation; extracellular matrix organization and biogenesis; regulation of neurotransmitter uptake; response to wounding; neurite regeneration; intermediate filament organization; astrocyte development

Disease: Alexander Disease