Western Blot (WB)
(Western blot analysis of Gelsolin on different lysates using anti-Gelsolin antibody at 1/500 dilution. Positive control: Lane 1: Mouse thymus Lane 2: Mouse lung Lane 3: THP-1 Lane 4: HepG2)
Rabbit anti-Human, Mouse Gelsolin Monoclonal Antibody | anti-GSN antibody
Gelsolin Antibody
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
Western Blot (WB)
(Western blot analysis of Gelsolin on different lysates using anti-Gelsolin antibody at 1/500 dilution. Positive control: Lane 1: Mouse thymus Lane 2: Mouse lung Lane 3: THP-1 Lane 4: HepG2)
Western Blot (WB)
(Western blot analysis of Gelsolin on different lysates using anti-Gelsolin antibody at 1/500 dilution. Positive control: Lane 1: Mouse thymus Lane 2: Mouse lung Lane 3: THP-1 Lane 4: HepG2)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Gelsolin antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Gelsolin antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Gelsolin antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Gelsolin antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Gelsolin antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Gelsolin antibody. Counter stained with hematoxylin.)
Immunocytochemistry (ICC)
(ICC staining Gelsolin in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining Gelsolin in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining Gelsolin in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining Gelsolin in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of THP-1 cells with Gelsolin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of THP-1 cells with Gelsolin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene binds to the "plus" ends of actin monomers and filaments to prevent monomer exchange. The encoded calcium-regulated protein functions in both assembly and disassembly of actin filaments. Defects in this gene are a cause of familial amyloidosis Finnish type (FAF). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
Gelsolin: a calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Its expression is significantly decreased in many tumors and transformed cell lines. Gelsolin and other proteins including E-cadherin, BRCA, p21WAF, HoxA5, and retinoic acid receptor II are regulated by epigenetic changes in breast cancers. Gelsolin helps mediate the ability of HDAC inhibitors to protect neurons from oxygen/glucose deprivation. Two human isoforms are produced by alternative initiation.
Protein type: Nuclear receptor co-regulator; Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis; Actin-binding
Chromosomal Location of Human Ortholog: 9q33
Cellular Component: ruffle; focal adhesion; protein complex; perinuclear region of cytoplasm; lamellipodium; extracellular region; cytosol; actin cytoskeleton
Molecular Function: protein binding; calcium ion binding; actin binding
Biological Process: regulation of cell adhesion; actin filament severing; phosphoinositide-mediated signaling; apoptosis; actin nucleation; vesicle-mediated transport; response to ethanol; actin filament polymerization; tissue regeneration; response to folic acid; oligodendrocyte development; barbed-end actin filament capping; cell structure disassembly during apoptosis; aging
Disease: Amyloidosis, Finnish Type
Research Articles on GSN
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Product Notes
The GSN gsn (Catalog #AAA9436565) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Gelsolin Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Gelsolin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF),Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:500-1:2000 IHC: 1:50-1:200 ICC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the GSN gsn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Gelsolin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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