Mouse GEF-H1 Monoclonal Antibody | anti-GEF-H1 antibody

GEF-H1, Human, mAb B4/7

Cat. Num. AAA584099

• Reactivity: Reactivity: Human. Cross reactivity: Canine
• Applications: Immunofluorescence, Immunoprecipitation, Western Blot
• Synonyms: GEF-H1; Monoclonal Antibody; Human; mAb B4/7; Rho guanine nucleotide exchange factor 2 (ARHGEF2); Microtubule-regulated Rho-GEF; Proliferating cell nucleolar antigen p40; KIAA0651; LFP40; anti-GEF-H1 antibody

• Host: Mouse
• Reactivity: Reactivity: Human. Cross reactivity: Canine
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Form/Format: 1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.; For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)

• Applicable Applications for anti-GEF-H1 antibody: F, Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot
• Application Notes: Application Notes: W: A non-reduced and/or reduced sample treatment and 6-15 % gradient SDS-PAGE was used. The band size is 110 kDa (Ref1-3).; Application Use: For immunohistochemistry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
• Immunogen: Protein fraction isolated from detergent extracts of MDCK cells1.
• Positive Control: MDCK cells
• Preparation and Storage: Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

Related Product Information for anti-GEF-H1 antibody

Product Description: The monoclonal antibody B4/7 recognizes human and canine guanine nucleotide exchange factor H1 (GEF-H1). GEFH1 is an ~110 kDa protein belonging to the Dbl family of proto-oncogenes. GEF-H1 can activate the small GTPase RhoA, but not Rac1 or Cdc42. Rho family GTPases are central regulators of epithelial tight junctions and the cytoskeleton. GEF-H1 can associate with different cytoskeletal structures, namely microtubules and the actin cytoskeleton. It has also been proposed to mediate crosstalk between the two types of filaments.
Localization of GEF-H1 differs between cell types. In MRC-5 fibroblast cells, GEF-H1 localizes to stress fibers. In epithelial cells, GEF-H1 is associated with apical tight junctions and involved in regulating paracellular permeability of small hydrophilic tracers. Furthermore, its subcellular localization changes in mitotic cells, where endogenous GEF-H1 is concentrated at mitotic spindles. GEF-H1 is capable of binding to the F-actin binding junctional adaptor cingulin. Binding to cingulin inhibits GEF-H1 and results in the downregulation of RhoA and inhibition of G1/S phase transition. In low confluent cultured cells, the localization of GEF-H1 is predominantly cytoplasmic. With increasing density of the cells, free GEF-H1 is sequestered at the tight junctions by cingulin.
GEF-H1 is part of the signaling pathway connecting epithelial polarity with the cell cycle, and as such involved in oncogenesis.

References

1. Benais-Pont, G et al; Identification of a tight junction-associated guanine nucleotide exchange factor that activates Rho and regulates paracellular permeability. J Cell Biol 2003, 160: 729 2. Aijaz, S et al; Binding of GEF-H1 to the tight junction-associated adaptor cingulin results in inhibition of Rho signaling and G1/S phase transition. Dev Cell 2005, 8: 777
3. Saito, Y et al; Conversion of Helicobacter pylori CagA from senescence inducer to oncogenic driver through polarity-dependent regulation of p21. J Exp Med 2010, 207: 2157
4. Tonami,K et al. Calpain-6, a microtubule-stabilizing protein. Regulates Rac1 activity and cell motility through interaction with GEF-H1. J of Cell science 2011, 124:1214-1223