Mouse GCA Monoclonal Antibody | anti-GCA antibody

GCA (Grancalcin, EF-hand Calcium Binding Protein, GCL) (HRP)

Cat. Num. AAA6182787

• Gene Names: GCA; GCL
• Applications: ELISA
• Purity: Purified
• Synonyms: GCA; Monoclonal Antibody; GCA (Grancalcin; EF-hand Calcium Binding Protein; GCL) (HRP); Grancalcin; GCL; anti-GCA antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 3D12
• Specificity: Recognizes GCA.
• Purity/Purification: Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
• Sequence Length: 217

• Applicable Applications for anti-GCA antibody: ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: GCA (AAH05214, 1aa-217aa) full-length recombinant protein with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MAYPGYGGGFGNFSIQVPGMQMGQPVPETGPAILLDGYSGPAYSDTYSSAGDSVYTYFSAVAGQDGEVDAEELQRCLTQSGINGTYSPFSLETCRIMIAMLDRDHTGKMGFNAFKELWAALNAWKENFMTVDQDGSGTVEHHELRQAIGLMGYRLSPQTLTTIVKRYSKNGRIFFDDYVACCVKLRALTDFFRKRDHLQQGSANFIYDDFLQGTMAI
• Conjugate: HRP
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(Detection limit for recombinant GST tagged GCA is 0.03 ng/ml as a capture antibody.)

Product Categories/Family for anti-GCA antibody

Antibodies; Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 13528828
• NCBI GeneID: 25801
• NCBI Official Full Name: Grancalcin, EF-hand calcium binding protein
• NCBI Official Synonym Full Names: grancalcin
• NCBI Official Symbol: GCA
• NCBI Official Synonym Symbols: GCL
• NCBI Protein Information: grancalcin
• Protein Family: Grancalcin

NCBI Description

This gene encodes a calcium-binding protein that is abundant in neutrophils and macrophages. In the absence of divalent cation, this protein localizes to the cytosolic fraction; with magnesium alone, it partitions with the granule fraction; and in the presence of magnesium and calcium, it associates with both the granule and membrane fractions. Alternative splicing and use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Aug 2016]