Mouse anti-Human GCA Monoclonal Antibody | anti-GCA antibody

GCA (Grancalcin, GCL) (FITC)

Cat. Num. AAA6147344

• Gene Names: GCA; GCL
• Reactivity: Human
• Applications: ELISA, Immunofluorescence, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GCA; Monoclonal Antibody; GCA (Grancalcin; GCL) (FITC); anti-GCA antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 2F5
• Specificity: Recognizes human GCA.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).

• Applicable Applications for anti-GCA antibody: ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: IF: 10ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Full-length recombinant corresponding to aa1-218 from GCA (AAH05214) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MAYPGYGGGFGNFSIQVPGMQMGQPVPETGPAILLDGYSGPAYSDTYSSAGDSVYTYFSAVAGQDGEVDAEELQRCLTQSGINGTYSPFSLETCRIMIAMLDRDHTGKMGFNAFKELWAALNAWKENFMTVDQDGSGTVEHHELRQAIGLMGYRLSPQTLTTIVKRYSKNGRIFFDDYVACCVKLRALTDFFRKRDHLQQGSANFIYDDFLQGTMAI*
• Conjugate: FITC
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (49.98kD).)

Western Blot (WB)

(GCA monoclonal antibody. Western Blot analysis of GCA expression in MCF-7.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to GCA on MCF-7 cell. [antibody concentration 10ug/ml].)

Testing Data

(Detection limit for recombinant GST tagged GCA is 0.03ng/ml as a capture antibody.)

Product Categories/Family for anti-GCA antibody

Antibodies; Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 13528827
• NCBI GeneID: 25801
• Molecular Weight: 24,010 Da
• NCBI Official Full Name: Homo sapiens grancalcin, EF-hand calcium binding protein, mRNA
• NCBI Official Synonym Full Names: grancalcin
• NCBI Official Symbol: GCA
• NCBI Official Synonym Symbols: GCL
• NCBI Protein Information: grancalcin
• Protein Family: Grancalcin

NCBI Description

This gene encodes a calcium-binding protein that is abundant in neutrophils and macrophages. In the absence of divalent cation, this protein localizes to the cytosolic fraction; with magnesium alone, it partitions with the granule fraction; and in the presence of magnesium and calcium, it associates with both the granule and membrane fractions. Alternative splicing and use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Aug 2016]