Mouse GBP2 Monoclonal Antibody | anti-GBP2 antibody

GBP2 antibody

Cat. Num. AAA831367

• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: GBP2 antibody was purified by affinity chromatography.
• Synonyms: GBP2; Monoclonal Antibody; GBP2 antibody; Monoclonal GBP2; Anti-GBP2; GBP 2; GBP-2; anti-GBP2 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 3B8
• Purity/Purification: GBP2 antibody was purified by affinity chromatography.
• Form/Format: Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 500 ug-1 mg/ml (varies by lot)
• Sequence Length: 591

• Applicable Applications for anti-GBP2 antibody: Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IF: 1:100; IHC: 1:50; WB: 1:1000
• Biological Significance: GBPs are characterized by their ability to specifically bind guanine nucleotides (GMP, GDP, and GTP). GBP2 is a GTPase that converts GTP to GDP and GMP.
• Cross-Reactivity: Human
• Immunogen: GBP2 antibody was raised in mouse using a protein expressed in 293T cell transfected with human GBP2 expression vector as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant GBP2 protein (Right) or empty vector (Left) detected with GBP2 antibody)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of GBP2 protein in paraffin embedded Adenocarcinoma of Human breast tissue using GBP2 antibody)

Related Product Information for anti-GBP2 antibody

Mouse monoclonal GBP2 antibody

Product Categories/Family for anti-GBP2 antibody

Proteases; Inhibitors; & Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 48146241
• NCBI GeneID: 2634
• UniProt Accession #: P32456; Q6GPH0; Q6IAU2; Q86TB0
• Molecular Weight: 67,209 Da
• NCBI Official Full Name: GBP2
• NCBI Official Synonym Full Names: guanylate binding protein 2, interferon-inducible
• NCBI Official Symbol: GBP2
• NCBI Protein Information: interferon-induced guanylate-binding protein 2
• UniProt Protein Name: Interferon-induced guanylate-binding protein 2
• Protein Family: Guanylate-binding protein
• UniProt Gene Name: GBP2
• UniProt Synonym Gene Names: GBP-2; HuGBP-2
• UniProt Entry Name: GBP2_HUMAN

NCBI Description

This gene belongs to the guanine-binding protein (GBP) family, which includes interferon-induced proteins that can bind to guanine nucleotides (GMP, GDP and GTP). The encoded protein is a GTPase which hydrolyzes GTP, predominantly to GDP. The protein may play a role as a marker of squamous cell carcinomas. [provided by RefSeq, Jul 2013]

Uniprot Description

GBP2: a member of the family of large GTP-binding proteins (GBPs) that is inducible by interferon. Possesses a high GTP hydrolysis activity. GBPs are the most abundant cellular proteins expressed in response to interferon-gamma (IFN-gamma). IFN-gamma, tumor necrosis factor-alpha (TNF-alpha), and interleukin-1beta (IL-1beta) strongly induce the expression of GBP-1, -2, and -3.

Protein type: Vesicle; Hydrolase

Chromosomal Location of Human Ortholog: 1p22.2

Cellular Component: Golgi membrane; cytoplasmic vesicle; nucleus; cytosol

Molecular Function: GTPase activity; protein binding; GTP binding

Biological Process: defense response to Gram-positive bacterium; metabolic process; cytokine and chemokine mediated signaling pathway; adhesion to host; immune response; defense response to protozoan