Mouse anti-Human Gasdermin D / GSDMD Monoclonal Antibody | anti-GSDMD antibody

Mouse Monoclonal [clone 3F12-1B2] (IgG1,k) to Human Gasdermin D / GSDMD

Cat. Num. AAA246242

• Gene Names: GSDMD; DF5L; DFNA5L; FKSG10; GSDMDC1
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, ELISA
• Purity: Protein A Purified
• Synonyms: Gasdermin D / GSDMD; Monoclonal Antibody; Mouse Monoclonal [clone 3F12-1B2] (IgG1; k) to Human Gasdermin D / GSDMD; Anti-Gasdermin D / GSDMD Antibody (clone 3F12-1B2) IHC-plus; GSDMD; DF5L; Gasdermin domain containing 1; FKSG10; Gasdermin D; DFNA5L; Gasdermin-D; GSDMDC1; Human Gasdermin D; anti-GSDMD antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 3F12-1B2
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2. Sourced in Ascites.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 484

• Applicable Applications for anti-GSDMD antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), ELISA (EIA)
• Application Notes: Immunohistochemistry-Paraffin (IHC-P): (5 ug/ml); Immunofluorescence (IF): (10 ug/ml).; (optimal dilution to be determined by the researcher)
• Target Species: Human
• Immunogen Description: GSDMDC1 (AAH08904, 1 a.a. ~ 485 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen Type: Recombinant protein
• Immunogen: Gasdermin D / GSDMD antibody was raised against gSDMDC1 (AAH08904, 1 a.a. ~ 485 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Anti-GSDMD / DFNA5L antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to GSDMDC1 on HeLa cell (antibody concentration 10 ug/ml).)

Western Blot (WB)

(GSDMDC1 monoclonal antibody, clone 3F12-1B2 Western blot of GSDMDC1 expression in Jurkat.)

ELISA (EIA)

(Detection limit for recombinant GST tagged GSDMDC1 is approximately 0.3 ng/ml as a capture antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 83320070
• NCBI GeneID: 79792
• NCBI Accession #: NP_079012.3
• NCBI GenBank Nucleotide #: NM_024736.6
• UniProt Accession #: P57764; Q96Q98; D3DWJ9
• Molecular Weight: 52,801 Da
• NCBI Official Full Name: gasdermin-D
• NCBI Official Synonym Full Names: gasdermin D
• NCBI Official Symbol: GSDMD
• NCBI Official Synonym Symbols: DF5L; DFNA5L; FKSG10; GSDMDC1
• NCBI Protein Information: gasdermin-D; gasdermin domain containing 1; gasdermin domain-containing protein 1
• UniProt Protein Name: Gasdermin-D
• Protein Family: Gasdermin
• UniProt Gene Name: GSDMD
• UniProt Synonym Gene Names: DFNA5L; GSDMDC1
• UniProt Entry Name: GSDMD_HUMAN

NCBI Description

Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described. [provided by RefSeq, Oct 2009]

Uniprot Description

FKSG10: Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described. [provided by RefSeq, Oct 2009]

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 8q24.3

Biological Process: cellular response to extracellular stimulus