Western Blot (WB)
(The extracts of mouse brain (50ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human GAS7 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Mouse anti-Human, Mouse GAS7 Monoclonal Antibody | anti-GAS7 antibody
GAS7 antibody
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Flow Cytometry, Functional Assay
Purity
By protein-G affinity chromatography
Synonyms
GAS7; Monoclonal Antibody; GAS7 antibody; Growth arrest-specific 7 isoform b; KIAA0394; Growth arrest-specific 7; isoform b Growth arrest specific 7; GAS 7; MGC1348; MLL/GAS7 fusion protein.; anti-GAS7 antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
AT4H8
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
416
Applicable Applications for anti-GAS7 antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500 ~ 1000.
Recommended starting dilution is 1:500.
Recommended starting dilution is 1:500.
Antigen Species
Human
Immunogen
Recombinant human GAS7 (1-416aa) purified from E. coli
Clone Note
Anti-human GAS7 mAb, clone AT4H8, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human GAS7 protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(The extracts of mouse brain (50ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human GAS7 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Western Blot (WB)
(The extracts of mouse brain (50ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human GAS7 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Immunofluorescence (IF)
(ICC/IF analysis of GAS7 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GAS7 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of GAS7 in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GAS7 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Immunofluorescence (IF)
(ICC/IF analysis of GAS7 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GAS7 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of GAS7 in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GAS7 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Related Product Information for anti-GAS7 antibody
Growth arrest-specific 7, also known as GAS7, is expressed primarily in terminally differentiated brain cells and predominantly in mature cerebellar Purkinje neurons. GAS7 plays a putative role in neuronal development by promoting maturation and morphological differentiation of cerebellar neurons. Inhibition of GAS7 production in terminally differentiating cultures of embryonic murine cerebellum impedes neurite outgrowth. Conversely, the hyper-expression of GAS7 may play an important role in the initiation and development of human osteosarcoma.
Product Categories/Family for anti-GAS7 antibody
References
Chao CC, et al., (2005) J Neurosci Res. 81 (2):153-62; She BR, et al., (2002) Exp Cell Res. 273 (1):34-44
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47,680 Da
NCBI Official Full Name
growth arrest-specific protein 7 isoform b
NCBI Official Synonym Full Names
growth arrest specific 7
NCBI Official Symbol
GAS7
NCBI Protein Information
growth arrest-specific protein 7
UniProt Protein Name
Growth arrest-specific protein 7
Protein Family
UniProt Gene Name
GAS7
UniProt Synonym Gene Names
KIAA0394; GAS-7
NCBI Description
Growth arrest-specific 7 is expressed primarily in terminally differentiated brain cells and predominantly in mature cerebellar Purkinje neurons. GAS7 plays a putative role in neuronal development. Several transcript variants encoding proteins which vary in the N-terminus have been described. [provided by RefSeq, Jul 2008]
Uniprot Description
May play a role in promoting maturation and morphological differentiation of cerebellar neurons.
Research Articles on GAS7
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Product Notes
The GAS7 gas7 (Catalog #AAA200146) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GAS7 antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GAS7 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS). WB: 1:500 ~ 1000. Recommended starting dilution is 1:500. Researchers should empirically determine the suitability of the GAS7 gas7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GAS7, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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