Mouse GAPDH Monoclonal Antibody | anti-GAPDH antibody

Anti-GAPDH Antibody (aa120-320, clone ABM22C5)

Cat. Num. AAA2401474

• Gene Names: GAPDH; G3PD; GAPD; HEL-S-162eP
• Reactivity: Human; Predicted: Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified
• Synonyms: GAPDH; Monoclonal Antibody; Anti-GAPDH Antibody (aa120-320; clone ABM22C5); Goat Polyclonal (IgG) to Human GAPDH; A1 40 kd subunit; Activator 1 40 kd subunit; G3PD; GAPD; G3pdh; Rfc40; Rf-c 40 kd subunit; anti-GAPDH antibody

• Host: Mouse
• Reactivity: Human; Predicted: Mouse
• Clonality: Monoclonal
• Isotype: IgG1, k
• Clone Number: ABM22C5
• Purity/Purification: Purified
• Form/Format: PBS, 0.05% sodium azide.
• Sequence Length: 335

• Applicable Applications for anti-GAPDH antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC-P: 10 ug/ml
• Immunogen: A partial length recombinant GAPDH protein (aa120-320).
• Conjugation: Unconjugated
• Epitope: aa120-320
• Preparation and Storage: Store at 4 degree C for short term.; Aliquot and store at -20 degree C for long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE))

Related Product Information for anti-GAPDH antibody

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylaseactivities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions areprobably due to the nitrosylase activity that mediates cysteineS-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton.

NCBI and Uniprot Product Information

• NCBI GI #: 7669492
• NCBI GeneID: 2597
• NCBI Accession #: NP_002037.2
• NCBI GenBank Nucleotide #: NM_002046.6
• UniProt Accession #: P04406; P00354; Q53X65; E7EUT4
• Molecular Weight: 31,548 Da
• NCBI Official Full Name: glyceraldehyde-3-phosphate dehydrogenase isoform 1
• NCBI Official Synonym Full Names: glyceraldehyde-3-phosphate dehydrogenase
• NCBI Official Symbol: GAPDH
• NCBI Official Synonym Symbols: G3PD; GAPD; HEL-S-162eP
• NCBI Protein Information: glyceraldehyde-3-phosphate dehydrogenase
• UniProt Protein Name: Glyceraldehyde-3-phosphate dehydrogenase
• UniProt Gene Name: GAPDH
• UniProt Synonym Gene Names: GAPD; GAPDH

NCBI Description

This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]

Uniprot Description

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.