Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Dyskeratosis Congenita 1 (DKC1) Standard Curve.)
Dyskeratosis Congenita 1 (DKC1) ELISA Kit | DKC1 elisa kit
Rat Dyskeratosis Congenita 1 (DKC1) ELISA Kit
Gene Names
Dkc1; Nap57
Reactivity
Rat
Synonyms
Dyskeratosis Congenita 1 (DKC1); Rat Dyskeratosis Congenita 1 (DKC1) ELISA Kit; DKC1; CBF5; DKC; FLJ97620; NAP57; NOLA4; XAP101; H/ACA ribonucleoprotein complex subunit 4; cbf5p homolog; dyskerin; nopp140-associated protein of 57 kDa; nucleolar protein family A member 4; snoRNP protein DK; DKC1 elisa kit
Reactivity
Rat
Specificity
Rat Dyskeratosis Congenita 1 (DKC1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat DKC1. No significant cross-reactivity or interference between Rat DKC1 and analogues was observed.
Sequence Length
509
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Dyskeratosis Congenita 1 (DKC1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Dyskeratosis Congenita 1 (DKC1) Standard Curve.)
Related Product Information for DKC1 elisa kit
Description: This Rat Dyskeratosis Congenita 1 (DKC1) ELISA Kit employs a two-site sandwich ELISA to quantitate DKC1 in samples. An antibody specific for DKC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DKC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DKC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DKC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Dyskeratosis congenita 1, dyskerin,is a member of the H/ACA snoRNPs (small nucleolar ribonucleoproteins). The H/ACA snoRNPs also include the NOLA1, 2 and 3 proteins. The protein encoded by this gene and the three NOLA proteins localize to the dense fibrillar components of nucleoli and to coiled (Cajal) bodies in the nucleus. Both 18S rRNA production and rRNA pseudouridylation are impaired if any one of the four proteins is depleted. The protein encoded by this gene is related to the Saccharomyces cerevisiae Cbf5p and Drosophila melanogaster Nop60B proteins. The gene lies in a tail-to-tail orientation with the palmitoylated erythrocyte membrane protein (MPP1) gene and is transcribed in a telomere to centromere direction. Both nucleotide substitutions and single trinucleotide repeat polymorphisms have been found in this gene.
Background: Dyskeratosis congenita 1, dyskerin,is a member of the H/ACA snoRNPs (small nucleolar ribonucleoproteins). The H/ACA snoRNPs also include the NOLA1, 2 and 3 proteins. The protein encoded by this gene and the three NOLA proteins localize to the dense fibrillar components of nucleoli and to coiled (Cajal) bodies in the nucleus. Both 18S rRNA production and rRNA pseudouridylation are impaired if any one of the four proteins is depleted. The protein encoded by this gene is related to the Saccharomyces cerevisiae Cbf5p and Drosophila melanogaster Nop60B proteins. The gene lies in a tail-to-tail orientation with the palmitoylated erythrocyte membrane protein (MPP1) gene and is transcribed in a telomere to centromere direction. Both nucleotide substitutions and single trinucleotide repeat polymorphisms have been found in this gene.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
H/ACA ribonucleoprotein complex subunit DKC1
NCBI Official Synonym Full Names
dyskerin pseudouridine synthase 1
NCBI Official Symbol
Dkc1
NCBI Official Synonym Symbols
Nap57
NCBI Protein Information
H/ACA ribonucleoprotein complex subunit DKC1
UniProt Protein Name
H/ACA ribonucleoprotein complex subunit 4
UniProt Gene Name
Dkc1
UniProt Synonym Gene Names
Nap57
UniProt Entry Name
DKC1_RAT
NCBI Description
nucleolar protein expressed in liver; colocalized with Nopp140 protein; may be involved with rRNA processing [RGD, Feb 2006]
Similar Products
Product Notes
The Rat DKC1 dkc1 (Catalog #AAA9712432) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712432 ELISA Kit recognizes Rat DKC1. It is sometimes possible for the material contained within the vial of "Dyskeratosis Congenita 1 (DKC1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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